摘要
目的:分离并测定黄连上清丸(片)(黄连,栀子,连翘,黄柏等)中的小檗碱型生物碱的含量。方法:采用反向高效液相法,以乙腈-3.4 g.L-1KH3PO4溶液(磷酸调pH为3.0)(40∶60),内含SDS1.7 g.L-1为流动相,345 nm检测。结果:在上述条件下,测定了10个厂家黄连上清丸(片)中小檗型生物碱(小檗碱、巴马汀、黄连碱、表小檗碱、药根碱)的含量。结论:本方法操作简便、结果准确、回收率高、重现性好,可用于该制剂的质量控制。
AIM： To separate and determine berberine type alkaloids in Huanglian Shangqing Pills （tablets） （Rhizoma Coptidis, Fructus Gardeniae, Fructus Forsythiae, Cortex Phellodendri Chinensis, etc. ）. METHODS： RP-HPLC was used, and the mobile phase consisted of acetonitrile-solution of potassium phosphate （3.4 g·L^-1, adjust pH to 3.0 with H3PO4） （40： 60）, with 1.7g·L^-1 SDS. The detecting wavelength was at 345 nm. RESULTS ： The contents of five kinds of berberine type alkaloids, berberine, palmatine, coptisine, epiberberine and jatrorrhizine in Huanglian Shangqing Pills （tablets） produced by 10 pharmaceutical factories were determined. CONCLUSION：The method is simple, accurate with a good recovery and repeatability. The method can be used to control the quality of Huanglian Shangqing Pills （Tablets）.
出处
《中成药》
CAS
CSCD
北大核心
2005年第12期1393-1396,共4页
Chinese Traditional Patent Medicine
基金
国家科技部重点科技攻关项目(99-929-01-07)
