摘要
目的获得中国大陆1b型丙型肝炎病毒(HCV)3′非编码区 (3′UTR)全长序列,探讨在丙型肝炎病毒3′非编码区(高变区)是否存在复杂的准种特性.方法利用逆转录套式聚合酶链反应(RT-PCR)限制性内切酶长度多态性分析(RFLP)初步筛选出6例1b型HCV感染者,采用半套式RT-PCR法扩增出约400 bp的cDNA片段,每例选择12～15个克隆测序.结果每例获得了5～8株全长1b型HCV3′非编码区克隆,由高变区,Poly(u)区,Poly(u/c)区及98碱基区四部分组成;各克隆中序列变异位点主要分布于高变区,Poly(u/c)区,核苷酸同源性0.2%～2.1%,呈现明显的准种分布特征.结论在丙型肝炎病毒3′非编码区(高变区)存在复杂的准种特性.
Objective To investigate the the quasispeeies character of 3＇ untranlated region（3＇ UTR） in hepatitis C virus（HCV） by analysing the nueleotide sequence polymorphism and mutation features in 3＇ UTR region, Methods Patients infected with genotype lb HCV were identified by reverse transcription -nested polymerase chain reaction （lit- PCR） and restriction fragment length polymorphism （RFLP） assay, Fragments of the eDNA of 3＇ UTR were amplified using semi -nested lit -PCR, and subjected to cloning. The 12 - 15 clones that contained HCV 3＇UTR gene fragments amplified from each patients were sequenced. Results The full - length sequence of l b genotype HCV 3＇UTR in eDNA were obtained. The 3＇UTR region consists of four elements ： the 5＇ region, the poly （U） , poly （U/C） and 98 - base region. The nueleotide sequence diversity ranged from 0. 2% - 2. 1% and the mutation points were almost distributed in the the 5 ＇region and poly（U/C）. Conclusions The HCV has complex quasispeeies character in 3＇ UTR.
出处
《中国医师杂志》
CAS
2005年第12期1598-1600,共3页
Journal of Chinese Physician
基金
国家自然科学基金(39770684
30170844)
国家"十五"科技攻关计划(2001BA705B06)资助
