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哮喘豚鼠气道内NK-1R mRNA表达及其含量的研究 被引量：5

Expression and content of neurokinin-1 receptor mRNA in the airway of asthmatic guinea pigs
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摘要 目的:研究哮喘豚鼠气道内神经激肽1受体(NK-1R)mRNA的分布及相对含量。方法:用卵蛋白超声雾化法制作哮喘豚鼠模型;原位杂交技术检测NK-1R mRNA,光镜观察其在气道内表达的部位。图像分析测定NK-1R mRNA的相对含量。结果:哮喘组NK-1R mRNA表达程度明显高于正常组;NK-1RmRNA分布于气管上皮细胞上层细胞表面、黏膜下层、血管周围上皮细胞、炎性细胞的表面和平滑肌细胞、腺体细胞表面。结论:NK-1R参与了卵蛋白诱导的哮喘豚鼠发病机制。 Objective： To study the expression and content of neurokinin-1 receptor（NK-1R） mRNA in the airway of asthmatic guinea pigs. Methods ：The model of asthmatic guinea pigs was established by aerosolized ovalbumin. The expression of NK-1R mRNA was detected by in situ hybridization, and the expression site was observed under the light microscope. The content of NK-1R mRNA was determined by image analysis. Results：The expression of NK-1 R mRNA in asthma group was higher than that in normal control group. NK-1R mRNA was expressed on the cell surface of tracheal epithelia, submucosa, blood vessels epithelia, inflammatory cells, smooth muscle ceils, and gland ceils. Conclusion：NK-1R may be involved in the pathogenesis of ovalbumin-induced asthma in guinea pigs.
作者 李淼 尚云晓
机构地区 中国医科大学附属第二医院儿科
出处 《中国医科大学学报》 CAS CSCD 北大核心 2005年第6期502-502,504,共2页 Journal of China Medical University
基金 国家自然科学基金资助项目(39900161)
关键词 哮喘 豚鼠 神经激肽1受体 asthma guinea pig neurokinin-1 receptor
分类号 R562.2 [医药卫生—呼吸系统]
  • 相关文献

参考文献7

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二级参考文献33

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共引文献22

同被引文献69

  • 1尚云晓,张晗,韩晓华,赵淑琴.糖皮质激素对哮喘豚鼠血浆、肺组织中神经激肽A含量的影响及其分子机制[J].中华儿科杂志,2005,43(6):418-420. 被引量：6
  • 2吕寒静,邱忠民,杨忠民,洪光朝,魏为利,王岚,余莉.气道速激肽在卵蛋白致敏豚鼠模型咳嗽反应中的作用[J].中国病理生理杂志,2005,21(9):1744-1747. 被引量：10
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引证文献5

二级引证文献28

中国医科大学学报
2005年 第6期

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