摘要
目的:观察补充支链氨基酸(BCAA)对划船运动员血液某些氨基酸浓度及血小板5-HT2A受体与螺环哌丁苯结合的影响,为运动员疲劳控制及合理营养补充提供实验依据。方法:18名男子划船运动员,随机分为安慰剂组、BCAA组及BCAA+CHO组,补剂的同时进行2周大运动量训练,2周后的1次急性运动前后,采用放射标记受体测定技术,测定各组运动员血小板5-HT2A受体与[3H]Spiperone(螺环哌丁苯)结合的最大结合容量、平衡解离常数的变化;同时测定血液BCAA、游离色氨酸的含量并进行分析。结果:(1)血浆中几种氨基酸浓度的变化:男子划船运动员2周大运动量训练后的1次急性运动前,各组安静值均无显著性差异;运动后BCAA组及BCAA+CHO组BCAA值与运动前相比显著提高。BCAA组和BCAA+CHO组运动员运动后血液丙氨酸浓度显著升高(P<0.05)。BCAA及BCAA+CHO组1次急性运动后即刻,fTrp/BCAA比值与运动前相比均显著降低。(2)血小板5-HT2A受体与[3H]Spiperone(螺环哌丁苯)结合的最大结合容量及平衡解离常数的变化:男子划船运动员2周大运动量训练后的1次急性运动后,安慰剂组较BCAA组和BCAA+CHO组最大结合容量下降更显著(P<0.05);BCAA组及BCAA+CHO组的平衡解离常数显著降低(P<0.05)。结论:2周大运动量训练期间补充BCAA或BCAA+CHO对改善血液BCAA含量、fTrp/BCAA比率等某些外周以及中枢疲劳指标有一定作用。
Objective To investigate the effect of supplementation of branched - chain amino acids （BCAA） on the concentration of some amino acid in blood and the binding capacity of platelets 5 - HT2AR with [^3H]Spiperone in rowers. Methods 18 male rowers were selected as subjects in this study and their plasma free tryptophan （fTrp）, alanine, phenylalanine, BCAA and 5 - HT2A R density of specific binding sites （Bmax） and affinity of [^3H]Spiperone for specific binding sites （Ka） on membranes from platelets were tested using radiolabeled receptor assay and biochemical techniques during 2 - week intensive training. Results There were significant decrease in plasma fTrp/BCAA and significant increase in BCAA and alanine in subjects supplied with BCAA （or BCAA ＋ CHO） during 2 - week intensive training （ P 〈 0.05）. Significant decreases in 5- HT2AR density of specific .binding sites （Bmax） and affinity of [^3H] Spiperone for specific binding sites （Kd） on membranes from platelets were found after acute intensive exercise. Bmax values of membranes from platelets after exercise in subjects with long - term supplying BCAA ＋ CHO were significantly higher than that in placebo group （ P 〈 0. 05）. Conclusion The supplementation of BCAA or BCAA ＋ CHO could slow down the decrement of 5 - HT2A and improve the plasma BCAA, fTrp/BCAA during long - term intensive exercise.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2005年第6期655-658,共4页
Chinese Journal of Sports Medicine
