摘要
目的:分离人CD4+CD25+Treg细胞,并进行功能分析。方法:应用免疫磁珠分离正常人外周血中CD4+CD25+Treg细胞,流式细胞仪检测细胞内因子IL-4、IFN-γ和IL-10表达,与CD4+CD25-T细胞共同培养,加或不加入中和抗体抗IL-10和抗TGF-β,检测其抑制功能。结果:CD4+CD25+Treg细胞约占正常外周血中CD4+T细胞的6%(2.4%￣18.9%,n=15),主要合成IL-10,能够以依赖剂量和非依赖IL-10和TGF-β方式抑制CD4+CD25-T细胞的增殖。结论:CD4+CD25+Treg细胞是一群具有免疫抑制功能的T细胞亚群细胞。
Objective： To isolate and analyse the function of human CD4^＋CD25^＋ T regulatory （Treg） cells . Methods： Magnetic microbeads were adopted to isolate human CD4^＋ CD25^＋ Treg cells. The levels of IL-4,IFN-Y and IL-10 were detected by flow cytometry. To assess the properties, CD4^＋ CD25^＋ Treg cells were cultured with CD4^＋ CD25-T cells either in the presence of neutralizing anti-human IL-10 or anti-human TGF-μ antibodies. Results： CD4^＋CD25^＋ Treg cells accounted for about 6% （2.4%-18.9%, n = 15） of CD4^＋ T cells in the peripheral blood of the healthy people. These cells mainly synthesized IL-10,and suppressed the proliferation of CD4^＋ CD25-T cells in a dose-dependent and IL-10 and TGF-μ independent manner. Conclusion： CD4^＋ CD25^＋ Treg cells are a subset of CD4^＋ T cells with immunosuppressive function.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第12期872-875,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省现代病原生物学重点实验室开放课题(XDBY04002)
南京医科大学创新基金资助项目(Cx200006)
