摘要
目的:观察NK1受体在大鼠牙髓及牙周组织中的表达情况。方法:采用冰冻切片和ABC免疫组化方法,检测NK1受体在大鼠牙髓及牙周组织中的表达。结果:NK1受体在大鼠牙周及牙髓组织内分布广泛,在牙周膜成纤维细胞、成牙骨质细胞、成牙本质细胞均呈强阳性表达;在牙髓成纤维细胞和牙槽骨细胞呈弱阳性表达;在牙齿根髓和冠髓内可发现染色阳性的血管样条索。结论:P物质可能直接作用于牙髓和牙周组织,细胞在牙髓及牙周炎症、修复反应中发挥作用。
Objective To investigate the localization of Neurokinin-1 receptor in rat dental pulp and periodontal tissues. Methods Neurokinin-1 receptor expression in dental pulp and periodontal tissues was detected by immunohitochemical method on frozen sections. Results Immunoreactivities for Neurokinin-1 receptor were distributed widely in dental pulp and periodontal tissues.There were strong immunoreactivities in the cytoplasm of odontoblasts,cemetoblasts,periodontal fibroblasts,and weak immunoreactivities in pulpal fibroblasts,alveolar osteocyts;Positive vessel-like structures were also found in pulp. Conclusion The findings further support the hypothesis that Substance P may have regulatory effects on pulp and periodontal tissues directly via neurokinin-1 receptors.
出处
《中国美容医学》
CAS
2005年第6期672-673,i0003,共3页
Chinese Journal of Aesthetic Medicine
基金
国家自然科学基金资助项目(编号30200320)
关键词
NK1受体
P物质
牙髓
牙周组织
neurokinin-1 receptor
substance P
pulp
periodontal tissues
