摘要
目的:面神经损伤修复的“金标准”是自体神经移植,但自体神经移植将不可避免造成患者的二次损伤而使其在临床的应用受到限制。通过观察神经干细胞移植修复面神经损伤的效果以探讨其可行性。方法:实验于2004-07/2005-06在郑州大学基础医学院解剖教研室神经生物学研究室完成。20只健康日本大耳白兔,随机分为两组,每组10只。治疗组:神经干细胞移植+内置胶原蛋白海绵的硅胶管;对照组:无细胞只有内置胶原蛋白海绵的硅胶管。术后12周,进行系列神经电生理检测、神经组织学观察、BrdU和S100免疫组织化学检测等检查。结果:实验兔20只均进入分析。①术后12周,神经电生理检测治疗组神经肌肉动作电位的潜伏期明显短于对照组[治疗组(1.65±0.21)m s,对照组(2.93±0.44)ms,P<0.01],而治疗组神经肌肉动作电位的波幅明显高于对照组[治疗组(6.1±0.35)m V,对照组(1.9±0.46)mV,P<0.01]。②术后12周,行BrdU单标、BrdU和S100双标免疫组织化学检测显示,治疗组有大量BrdU阳性细胞,且部分阳性细胞同时呈现S100双标阳性。对照组未见BrdU阳性细胞。③术后12周,半薄和超薄切片显示治疗组的再生纤维以有髓神经纤维为主,髓鞘板层结构清晰,轴浆内细胞器丰富。对照组的再生纤维髓鞘发育差,但能看到典型的髓板层结构,轴浆内细胞器较少。结论:神经干细胞移植能显著提高面神经损伤后修复的效果。
AIM： Autologous nerve transplantation is the“gold standard”for the repair of facial nerve injury, but it will unavoidably result in the second injury, which restrict its application in clinic. The paper observed the effect on repairing the facial nerve defect of rabbits by implanting neuronal stem cells so as to investigate its feasibility. METHODS： The experiment was carried out in the neurobiological laboratory of the Department of Anatomy, College of Basic Medical Sciences, Zhengzhou University between July 2004 and June 2005. Twenty healthy Japanese big-ear white rabbits were randomly divided into 2 groups with 10 rabbits in each group： treatment group （silicone guidance channel with collagen protein sponge were filled with neuronal stem Cells） and control group （silicone guidance channel with collagen protein sponge）. After 12 weeks, a series of examinations were performed, including neuroelectrophysiological methods, neurohistological staining, BrdU and S100 immunohistochemical staining. RESULTS： All the 20 rabbits were involved in the analysis of results. ① At 12 weeks after operation, the neuroelectrophysiological examinations revealed that the latency of nerve and muscle action conduction in the treatment group was shorter than that in the control groups [（1.65±0.21） ms, （2.93±0.44） ms, P〈0.01], but the wave amplitude of nerve and muscle action conduction in the treatment group was obviously higher than that in the control group [（6.1±0.35） mV, （1.9±0.46） mV, P〈0.01].② At 12 weeks after operation, the immunohistochemical examination of BrdU single labeling and BrdU and S100 double labeling showed that there were a great deal of BrdU positive cells and part of them also showed S100 double labeled positive, while no BrdU positive cell was observed in the control group.③ At 12 weeks after operation, the semithin section and ultrathin section showed that the regenerated fibers in the treatment group were mainly myelinated nerve fiber, the layer structure of myelin sheath was clear, and there were rich organells in axoplasma, but the myelin sheath of myelinated nerve fiber developed worse in the control group, but typical layer structure of myelin sheath could be observed, and there were fewer organells in axoplasma. CONCLUSION： Transplant of neuronal stem cells can improve the effect of repairing facial nerve injury.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第42期6-8,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家教育部"十五""211"工程重点学科"肿瘤与生物工程"项目[教重字(2002)第2号]~~
