摘要
目的探讨环氧化酶2(COX2)抑制剂尼米舒利(NIM)与化疗药依托泊苷(VP16)联用对肺癌细胞增殖和凋亡的影响。方法培养人肺癌细胞A549:①NIM(25μmol/L)与VP16(2～32μg/mL)联合,MTT试验观察干预48h后细胞增殖变化及增殖抑制率;②分为对照组、NIM组(25μmol/L)、VP16组(8μg/mL)和NIM+VP16组,观察12h、24h及48h后A549细胞生长情况,描记生长曲线;流式细胞术检测干预48h后细胞凋亡率。结果①VP16能抑制肺癌A549细胞的增殖,且呈量效关系(r=-0.908,P<0.01),NIM与VP16联用后,抑增殖作用增强,二者有相加或协同作用;②NIM及VP16均可抑制A549细胞的生长,诱导凋亡,二者联用后作用增强。结论NIM与VP16联用可增强对肺癌细胞增殖的抑制和凋亡的诱导,二者具有协同抗肿瘤作用。
Objective To investigate the effects of cyclooxygenase-2 selective inhibitor nimesulide （NIM） combined with etoposide （VP-16） on cell proliferation and apoptosis in human lung cancer. Methods Human lung cancer A549 cells were incubated and the studies were done as follows： ①NIM （25μmol/L） combined with VP-16 （2-32μg/mL） coincubated with A549 cells for 48h, the cell proliferation status and proliferation inhibition rate were detected by MTT reduction assay. ②The cells were divided into control group, NIM （25/μmol/L） group, VP-16 （8μg/mL） group and NIM ＋ VP-16 group. The cells growth activity was observed by growth curve as cells coincubated for 12h, 24h, 48h, and the cell apoptosis rate was analyzed by flow cytometry. Results ①VP-16 could inhibit A549 cells proliferation in a concentration dependent pattern, which was enhanced as combined with NIM. NIM and VP-16 acted in a synergistic or additive pattern. ②NIM and VP-16 could induce A549 cells apoptosis effectively and the antitumor effects were augmented as they combined. Conclusion NIM combined with VP-16 can enhance the effects of growth inhibition and apoptosis induction and they play a synergistic anti-tumor effect in lung cancer cell.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2005年第11期689-691,共3页
Cancer Research on Prevention and Treatment
关键词
肺癌
凋亡
增殖
环氧化酶-2
依托泊苷
Lung neoplasm
Apoptosis
Proliferation
Cyclooxygenase 2
Etoposide
