摘要
简介人红细胞氧耗率的薄膜氧电极检测技术。测得我国１２名正常成人红细胞的内源性氧耗率为０．０６６±０．０３０（６男）和０．１１３±０．０６８μｌ／（ｈ·ｍｇ干重）（６女），明显高于测压法（Ｐ＜０．０１）。１２名正常成人红细胞对葡萄糖的利用［氧耗车０．４５２±０．０６９μｌ／（ｈ·ｍｇ干重）］与测压法结果相近（Ｐ＞０．０５）。本法具有主观性好、简便和较灸敏的优点。
ulfilling the requirements of blood biochemistry,techniques for estimation of the oxygenconsumption rate in human erythrocytes by thin-membrane oxygen electrode were established.Inthis paper,the constitution,operational procedures and result calculation of the measuring sys-tem are briefly described.The endogenous respiration of erythrocytes in 12 healthy Chineseadults was 0.066±0.030(6 males)and O20.113±0.068μl/(h·mg dry weight)(6 females)respectively,which were significantly higher than those obtained by the manometric method(P<0.01).The utilization of glucose by the erythrocytes of 12 healthy Chinese adults[(oxygenconsumption rates 0.452±0.069μl /(h· mg dry weight)] was very close to the figures ofmanometric method( P >0.05)].Therefore,this method is simple, rapid,considerably sensi-tive and well reproducible(CV=6.73%).
出处
《湖南医科大学学报》
CSCD
1996年第1期78-80,共3页
Bulletin of Hunan Medical University
基金
中华医学基金
关键词
氧电极技术
红细胞
氧耗率
oxygen electrode techniques
human
erythrocytes
oxygen consumptionrate:methods①Item pertaining to a project supported by Chinese Medlcal Board(CMB)Grant(N.,900105)
