摘要
目的:观察养肝利胆颗粒对小鼠四氯化碳(carbon tetrachloride,CCl4)肝损伤的影响,并探讨其作用机制。方法:采用腹腔注射CCl4制作小鼠慢性肝损伤模型。造模3周后分别予以养肝利胆颗粒小、中、大剂量,胆宁片及联苯双酯治疗4周,检测各组血清丙氨酸氨基转移酶(alanine aminotransferase,ALT),天门冬氨酸氨基转移酶(aspartate aminotransferase,AST),肝组织超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde,MDA)含量。结果:养肝利胆颗粒可显著降低小鼠CCl4肝损伤模型血清ALT、AST的水平和肝组织MDA的含量,明显升高肝组织SOD的水平。结论:养肝利胆颗粒对CCl4所致小鼠肝损伤有明显的改善作用,其作用机制可能与减轻肝内脂质过氧化反应有关。
Objective： To observe the protective effects of Yanggan Lidan Granules （YGLDG） on carbon tetrachloride （CCI4）-induced liver damage in mice and to find out its mechanism. Methods. A model of chronic liver damage was established in mice by intraperitoneal injection of CCI4. After three weeks, those model mice were treated with low-, medium-, high-dose YGLDG, Danning Tablets and bifendate respectively for four weeks. Then the levels of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST）, as well as the contents of superoxide dismutase （SOD） and malondialdehyde （ME）A） in liver tissues were detected. Results： YGLDG could significantly reduce the levels of serum ALT and AST in model mice, and the content of MDA was obviously decreased while the content of SOD was increased in liver tissue. Conclusion： The therapeutic effect of YGLDG on mice with CCI4-induced liver damage is to relieve the seriousness of liver damage, and its mechanism may relate to reducing peroxidation activity in liver tissue.
出处
《中西医结合学报》
CAS
2005年第6期470-472,共3页
Journal of Chinese Integrative Medicine
基金
上海市科学技术发展基金项目中药现代化专项资助项目(No.014319304)
上海市重点学科中医外科资助项目(No.01ZX004)
关键词
养肝利胆颗粒
四氯化碳
超氧化物歧化酶
丙二醛
Yanggan Lidan Granules
carbon tetrachloride
superoxide dismutase
malondialdehyde
