摘要
应用电子显微技术,对山楂粉蝶核型多角体病毒感染三龄幼虫后,该病毒在体内的形态发生过程及其宿主细胞病理超微结构的变化等进行了观察与分析。结果表明:病毒感染72~168h后,山楂粉蝶幼虫中肠上皮细胞内出现病毒发生基质、核衣壳、套膜、丝状纤维或称前多角体蛋白。病毒粒子、病毒束,以及病毒束嵌入多角体蛋白,装配成完全的病毒多角体。在相应的中肠上皮细胞内,细胞器如线粒体、粗面内质网、核糖体、溶酶体等均发生显著的病理变化。同时还应指出的是内质网呈指纹图谱型,板层体与示髓样小体等膜状结构的病理变化比较特殊。
he morphogenetic process of Acr NPV in vivo and cytopathology of its host cells were ob-served and analysed using electron micloscopy techniques after Acr NPV infected larval stagehost.The investigation shows:after innoculation 72~168h,profuse virogennic stroma,nucleocap-sid.envelope,filament,polyhedrirn,virion,viral bundle and complete Acr NPV existed withinmidgut epithelia cells.The cell organs have apparent pathological changes. Endoplasmic reticuiumtakes the form of fingerprint type,and there are a lot of myloid bodies.
出处
《中国病毒学》
CSCD
1996年第2期138-143,共6页
Virologica Sinica
关键词
昆虫病毒
山楂粉蝶
核型多角体病毒
形态
病理
Aporid crataegi NPV,Morphogensis,Cell organ,Ultrastructure,Pathological change