期刊文献+

海因酶热稳定性及底物特异性研究进展 被引量:3

Progress in study on thermostability and substrate specificity of hydantoinase
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摘要 海因酶是在微生物中广泛分布的能水解5-取代海因衍生物制备光学纯氨基酸的关键生物催化剂,在各种氨基酸的酶法生产中具有良好的应用前景。着重概述了海因酶的热稳定性、底物特异性研究及应用,并讨论了其发展方向。 Hydantoinase catalyses the cleavage of the ring of DL-5-monosubstituted hydantoins, which is the key bioeatalyst involved in the production of chiral amino acids and is widely found in microbial world. The thermostability and substrate specificity of hydantoinase were briefly revieweol in the article, and the develapment trend was discassed.
出处 《生物加工过程》 CAS CSCD 2005年第3期24-28,37,共6页 Chinese Journal of Bioprocess Engineering
基金 国家973项目(2003CB716000)
关键词 海因酶 热稳定性 底物特异性 hydantoianse thermostability substrate specificity
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参考文献37

  • 1[1]张炳荣.氨基酸工业大全[M].北京:中国轻工业出版社,1991.
  • 2[2]Margolin AL.Enzymes in the synthesis of chiral drugs[J].Enzyme Microb Technol,1993,15(4):266-280.
  • 3[3]Stephanic GB,Rosemary AD.Hydntoin-hydrdysing enzymes for the enantioselective production of amino acids:new insights and applications[J].Tetrohedron:asymmetry,2004,15:2 227-2 741.
  • 4[4]Buchanan K,Burton SG,Dorrington RA,et al.A novel Pseudomonas putida strain with high levels of hydantoin-converting activity,producing L-amino acids[J].J Molecular catalysis B,2001,11:397-406.
  • 5姚忠,朱姝,李家璜,屠春燕,李苏平,韦萍.海因酶/N-氨甲酰水解酶催化DL-苄基海因制备D-苯丙氨酸的过程分析及强化[J].现代化工,2004,24(5):44-46. 被引量:5
  • 6[6]Kim GJ,Kim HS.Optimization of the enzymatic synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin using D-hydantoinase and N-carbamoylase[J].Enzyme Microbial Technology,1995,17:63-67.
  • 7[7]Holst O,Manelius A,Krohe M,et al.Thermophiles and Fermentation Technology[J].Comp Biochem Physiol,1997,118(3):415-422.
  • 8[8]Yamada H,Takahashi S.Distribution of Hydantoin Hydrolyzing Activity in Microorganisms[J].J Ferment Technol,1978,56(5):484-491.
  • 9[9]Simann M,Syldatk C,Wagner F.Detection and comparison of strains with selective L-hydantoin cleaving activity using polyclonal antibodies[J].Biotechnol Tech,1993,7:361.
  • 10[10]Yokozeki K,Sano K,Eguchi C,et al.Enzymatic production of L-tryptophan from of DL-5-indolylethylhydantoin by mutants of Flavobacterium sp.T-523[J].Agric Biol Chem,1987,51(2):363-369.

二级参考文献24

  • 1Syldatk C, May O, Altenbuchner J, et al. Microbial hydantoinases--industrial enzymes from the origin of life [J]? Appl Mierobiol Biotechnol, 1999,51(3):293.
  • 2Abendroth J, Niefind If. May O, et al. The structure of L-hydantoinase from Arthobacter aurescerts leads to an understandirig of dihydropyrimidinase substrate and enantio specificity[J]. Biochemistry, 2002,41(27):8589.
  • 3Cheon YH, Kim HS, Han KH, et al. Crystal structure of D-hydantoinnse from Bacillus stecarothermophilus: insight into the stereochemistry of enantioselectivity [J]. Biochemistry, 2002,41(30):9410.
  • 4Abendroth J, Niefind K, Schomburg D. X-ray structure of a dihydropyrimidinase from Themuts sp. at 1.3A resolution[J]. J Mol Biol, 2002,320(1):143.
  • 5Sail A. Modeling mutations and homologous proteins [J]. Curt Opin Biotech, 1995,6:437.
  • 6Sali A. Protein modeling by satisfaction of spatial restraints[J].Molecular Medicine Today, 1995,1:270.
  • 7Benini S, Rypniewski WR, Wilson KS, et al. A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels[J]. Structure, 1999,7:205.
  • 8Thoden JB, Phillips GN Jr, Neal TM, et al. Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center[J]. Biochemistry, 2001, 40: 6989.
  • 9Nanba H,Biosci Biotechnol Biochem,1998年,62卷,5期,875页
  • 10Lee C K,Lee Z S,Yang P F. [J]. Enzyme Microb Technol,2001,28(9 - 10) :806 - 814.

共引文献22

同被引文献72

  • 1刘森林.水-有机相在制备D-对羟基苯甘氨酸中的应用[J].深圳大学学报(理工版),2004,21(3):262-265. 被引量:2
  • 2于平.海因酶研究新进展[J].生物学杂志,2005,22(2):1-4. 被引量:5
  • 3王婕,张惟材,刘党生.乙内酰脲酶及其在氨基酸手性合成中的应用[J].生物技术通讯,2005,16(5):567-570. 被引量:2
  • 4[1]Stephanic G B,Rosemary A D.Hydntoin-hydrolyzing enzymes for the enantioselective production of amino acids:new insights and applications[J].Tetrahedron:Asymmetry,2004,15:2227-2741.
  • 5[2]Buchanan K,Burton S G,Dorrington R A,et al.A novel Pseudomonas putida strain with high levels of hydantoin-converting activity,producing L-amino acids[J].J Molecular catalysis B,2001,11:397-406.
  • 6[7]Serge M R,Patrick C.Purification and biochemical characterization of the hydantoin hydrolyzing enzyme from Agrobacerium species A hydantoinase with no 5,6-dihydropyrimidine amidohydrolase activity[J].Biochem,1993,213:1315-1324.
  • 7[8]Park J H.Purification and characterization of thermostable D-hydantoinase from Bacillus thermocatenulatus GH-2[J].Appl Biochem Biotechnol,1999,81(1):53-65.
  • 8[9]Chung J H,Back J H,Park Y I,et al.Thermostable hydantoinase from a hyperthermophilic archaeon,Methanococcus jannaschii[J].Enzyme and Microbial Technology,2002,7(30):867-874.
  • 9[10]Wilms B,Wiese A.Cloning,nucleotide sequence and expression of a new L-N-carbamoylase gene from Arthrobacter aurescens DSM 3747 in E.coli[J].J Biotechnol,1999(68):101-113.
  • 10[11]Watabe K,Ishikawa T.Cloning and sequencing of the genes involved in the conversion of 5-substituted hydantoins to the corresponding L-amino acids from the native plasmid of Pseudomonas sp.strain NS671[J].J Bactenol,1992,3(174):962-969.

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