摘要
了解TGFβ1基因对血管内皮细胞表达细胞外基质蛋白及与基质黏附力的影响。用DOTAP脂质体转染p MAMneo TGFβ1于原代培养的脐静脉内皮细胞,经G4 18筛选,TGFβ1表达经免疫荧光鉴定。Western blot确定 型胶原、纤黏连蛋白的表达,微管吸吮系统确定内皮细胞与基质的黏附力。结果表明生理情况下的内皮细胞能表达少量的TGFβ1及胶原、纤黏连蛋白。经G4 18筛选,外源性TGFβ1在血管内皮细胞中稳定表达,能显著提高胶原、纤黏连蛋白纤维的表达及细胞与基质的黏附。说明TGFβ1在血管组织工程中促进内皮细胞的黏附具有一定的应用价值。
This study was amimed to examine the effectiveness of a gene transfer of human TGFβ1 gene into endothelial cells and to determine whether TGFβ1 increases ECM expression of endothelial cells. With the help of DOTAP, endothelial cells were transfected with pMAMneoTGFβ1. The positive cell clones were selected with G418. The stable transfection and expression of TGFβ1 in the endothelial cells were determined by immunofluorescence analysis. The expression levels of collagen I and fibronectin in the transfected and untransfected endothelial cells were determined by Western blot. The adhesion force between endothelial cells and matrix was determined by a micropipette technical system. The results showed abundant TGFβ1 stable expression in the endothelial cells. TGFβ1 gene was noted to increase collagen I and fibronectin expression and increase the adhesion between endothelial cells and matrix. These findings indicated that TGFβ1 can be used in vascular tissue engineering for the enhancement of endothelial cells adhesion.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2005年第2期271-274,共4页
Journal of Biomedical Engineering
基金
全军十五青年基金项目 (0 1Q0 98)
高等学校重点实验室访问学者基金资助
重庆市科学基金资助项目
