摘要
以野生白木香Rosa banksiaevar.normalis茎段为外植体,探讨了不同生长调节物质对外植体分化、增殖和生根的影响,并对栽培基质与移栽技术进行研究。结果表明,利于白木香分化的诱导培养基为MS+2.0 mg.L-16-BA+0.1 mg.L-1NAA;MS+2.0 mg.L-16-BA+0.05mg.L-1NAA作增殖培养基效果最好,增殖率为5.5;利于生根的培养基为1/2MS+0.2 mg.L-1IBA+0.2 mg.L-1NAA;移栽基质为蛭石+珍珠岩+腐叶土(1∶1∶1)效果最好,成活率98%,且苗生长健壮。建立了白木香高效再生体系。
Using wild Rosa banksiae var. normalis of explants, the effects of different plant growth regulators on differentiation, multiplication and rooting of explants, and transplant technique and cultivated material were studied. The results showed that the best medium for inducing was MS ＋ 2.0 mg· L^-1 6-BA ＋ 0.1 mg· L^- 1NAA ; that the best medium for the multiplication of medium was MS ＋ 2.0 mg· L^-1 6-BA ＋ 0.05 mg·L^-1 NAA, and the multiplication rate was 5.5; that the rooting and growing was the best on the medium with 1/2 MS ＋ 0.2 mg· L^-1 IBA ＋ 0.2 mg· L^-1 NAA. Plant material was the best with the vermiculite ＋ pearlite ＋ leaf mold （ 1 ： 1 ： 1 ）, and the survival rate was 98 %. High efficient regeneration system of wild Rose banksiae was established by using of wild Rosa banksiae var. normalis of eggplants. [ Ch, 4 tab. 10 ref. ]
出处
《浙江林学院学报》
CSCD
北大核心
2005年第4期410-413,共4页
Journal of Zhejiang Forestry College
基金
河南省南阳市科技攻关项目(200404016)
关键词
白木香
茎段
离体培养
植株再生
树状月季
Rosa banksiae var. normalis
stem shoots
vitro culture
plant regeneration
tree-rose
