摘要
目的:检测正常豚鼠内耳组织中水通道蛋白(aquaporins,AQPs)的表达,探讨其在内耳液体平衡中的意义。方法:用免疫组织化学方法,以兔抗大鼠AQP0、1、2、3、5、7、8的多克隆抗体,检测正常豚鼠内耳组织中水通道蛋白亚型0、1、2、3、5、7、8的表达。结果:水通道蛋白亚型0、1、2、3、5、7、8在豚鼠内耳有不同程度、不同模式的表达,其中AQP0仅在血管纹上皮细胞、螺旋神经节细胞有较弱的表达,AQP1的分布见于包绕骨迷路、内淋巴囊、内淋巴管的纤维细胞,基底膜鼓阶面细胞、螺旋韧带纤维细胞、螺旋缘纤维细胞、Corti器、内外螺旋沟、血管纹、椭圆囊壁、球囊壁、螺旋神经节细胞等。AQP2表达在血管纹、Corti器、螺旋神经节细胞和内淋巴囊中。AQP3、7、8的分布类似,在螺旋神经节和包绕膜迷路的组织中均有表达,其中Corti器、内外螺旋沟、血管纹、螺旋神经节表达较强,在螺旋韧带、螺旋缘纤维细胞表达较弱。AQP5则在Corti器、内外螺旋沟、螺旋神经节细胞表达较强,在螺旋韧带纤维细胞表达稍弱。结论:在正常豚鼠内耳中,尤其是膜迷路中有多种水通道蛋白亚型,以不同的方式表达,他们可能在维持膜迷路液体平衡中起着协同作用。
Objective：To investigate the expression and its significance of aquaporins （AQPs） in normal guinea pig inner ears. Method ： Ten healthy guinea pigs were used. Immunohiatochemistry were employed to detect AQP0, 1, 2, 3, 5, 7, 8 in normal guinea pig inner ears, using rabbit anti-rat polyclonal antibodies. Result： AQP0, 1, 2, 3, 5, 7, 8 were all expressed in guinea pig inner ears. AQP0 was only located in stria vascularis and spiral ganglion. The distribution of AQP1 consisted of cells lining the bony labyrinth, fibrocytes lining the endolymphatic duct and sac, cells under the basilar membrane, fibrocytes of the spiral ligament and the spiral limbus, Cortirs organ, inner and outer spiral sulcus, stria vascularis, saccular and utricular wall, and spiral ganglion. AQP2 located in stria vascularis, Corti＇s organ, spiral ganglion and endolymphatic sac. AQP3,7,8 distributed in a similar manner which was surrounding the membranous labyrihth, including Cortirs organ, inner and outer spiral sulcus, stria vascularis, fibrocytes of the spiral ligament and the spiral limbus,saccular and utricular wall,endolymphatic sac and spiral ganglion. AQP5 located at Cortirs organ, inner and outer spiral sulcus, spiral ganglion, fibrocytes in spiral ligment. Conclusion： Diverse AQPs are distributed in the normal guinea pig inner ears, which may co-work to maintain the homeostasis of the inner ear.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第19期883-885,共3页
Journal of Clinical Otorhinolaryngology
