摘要
硫酸乙酰肝素酶是迄今为止在哺乳动物细胞中发现的唯一可以剪切胞外和细胞表面硫酸乙酰肝素多糖侧链的葡糖苷酸内切酶.在恶性肿瘤、炎症细胞以及胚胎组织等具有侵袭性组织中有较高的表达,肿瘤病人病灶部位的肝素酶mRNA表达量越高,病人存活期越短.在正常生理条件下,肝素酶基因及其表达蛋白的活性受到启动子甲基化、变化转录剪切、转录因子、蛋白质加工、pH环境以及免疫因子释放等多种内源因素的精确调控,以防止机体非正常恶性变化的发生.目前就有关乙酰肝素酶基因表达调控、酶活性的调控机制作详尽的专述.
Heparanase is the only mammalian endo-β-D-glucuronidase known to cleave heparan sulfate （HS） components ofproteoglycans（PGs） at limited intra-chain sites. Heparanase release in response to an inflammatory stimulus or in relation to tumor metastasis alters the composition and structural integrity of extracellular matrix and basement membrane. The enzymatic degradation of HS by heparanase is, therefore, involved in range of biological phenomena, from pregnancy, morphogenesis and development to inflammation, vascularization, and cancer progression. In normal physiological processes, expression of the active enzyme is tightly regulated by promoter methylation, mRNA splicing, transcription factors, proteolytic processing and inflammatory cytokines. The recent findings about the strict regulation of heparanase product to yield the active enzyme are reviewed. from the expression of the gene to processing of the protein
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2005年第9期817-821,共5页
Progress In Biochemistry and Biophysics
基金
CRCG基金资助项目(10204997和10205634).~~
