摘要
线粒体DNA是研究动物系统发育、种群遗传变异与分化和进行近缘种、种下分类单元鉴定的理想工具之一。用改进的碱变性提取法对蝗虫线粒体DNA进行快速提取,结果表明:新鲜材料和-80℃冷藏的标本是提取mtDNA的理想材料,直接取运动肌(胸部和后足胫节肌肉)时每克组织所得到的mtDNA较多、较纯。
Mitochondrial DNA is widely used in the studying field of mechanism of heredity and breeding of animals,evolution of creatures.Alkaline lysis method is used to isolate and purify mtDNA of locust in this test.It is indicated that the fresh materials and samples deepfreezed at-80 ℃ are the ideality materials for extracting mtDNA.The quality and purification of mtDNA from sport muscle are high and suitable to all kinds of studies on mtDNA.
出处
《河南农业科学》
CSCD
北大核心
2005年第10期52-54,共3页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金重点资助项目(39630210)
