摘要
采用碱变性法制备绿头鸭肝细胞ｍｔＤＮＡ，经纯化后测其分子量为１６．７ｋｂ．用限制性内切酶进行酶切分析，结果表明ＢａｍＨⅠ、ｐｓｔⅠ和ＨｉｎｄⅢ在绿卖鸭ｎｔＤＮＡ分子上分别有４个、２个和３个酶切位点。与由番鸭、北京鸭和建昌鸭肝细胞ｍｔＤＮＡ为材料所得的研究结果比较，发现绿头鸭与番鸭及家鸭在ｍｔＤＮＡ种质方面存在着高度差异。
mtDNA from liver of Anas platyrhynchos was prepared by the alkaline lysisprocedure. Its molecular weight was measured to be 16.7 kb.According to analysis of restrictionenzyme cleavage,restrtction endonucleases BamHⅠ,Pst Ⅰ and Hind Ⅲ have 4, 2 and 3 sites onmtDNA from Anas platyrhynchos,respectively,mtDNAs of livers from Anas platyrhynchos,Cai-rina moschata and Anas domestica(Beijing duck and jianchang duck)have high heredity variationas compared with study results from Wu Heling。
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1995年第4期614-618,共5页
Journal of Xiamen University：Natural Science
基金
福建省自然科学基金
