摘要
The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches were obtained as follow : the cell suspending mixture obtained the highest enzyme activity(20.4U/mL),after being treated with by Triton X-100 and β-ME(5%,v/v) and ultrasonic.The enzyme activity obtained by this method was obviously higher than by single treating method,e.g.ultrasonic(7.6U/mL) or Triton X-100 and β-ME(15.2U/mL).
The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11. The results of our researches were obtained as follow ： the cell suspending mixture obtained the highest enzyme activity （20.4U/mL） ,after being treated with by Triton X-100 and β-ME （ 5% , v/v ） and ultrasonic. The enzyme activity obtained by this method was obviously higher than by single treating method, e.g. ultrasonic （7.6U/mL） or Triton X-100 and β-ME （15.2U/mL）.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第5期1057-1060,共4页
Journal of Sichuan University(Natural Science Edition)
关键词
产朊圆酵母
尿酸酶
破碎技术
细胞壁
提取方法
Torula utilis Henneb 2.281
combination of different extraction method
higher Uricase activity
higher extraction ratio
