摘要
用NAA、IBA处理樱桃砧木嫩枝和无根组培苗,测定了插条生根率、生根条数和生理、生化指标的动态变化。结果表明:经处理后插穗的各项生根指标都得到了显著提高,其中100 mg/L浓度的NAA与IBA处理插穗生根率达到了88.3%和85%,组织培养的试管苗瓶外生根率达89%和87.5%;嫩枝插穗的可溶性糖、可溶性蛋白质、叶绿素、核酸物质的含量与对照组相比发生了显著的变化:在不定根原基的诱导期,插穗叶绿素、核酸、可溶性糖含量显著增加,可溶性蛋白质含量下降;在不定根的形成期,插穗的可溶性糖被不定根的形成所消耗而含量显著下降,但可溶性蛋白质含量逐渐上升;在不定根形成后插穗具有了吸收外界营养的能力,故在不定根伸长期叶片中可溶性糖开始积累,含量上升。这些物质含量的动态变化与插穗生根相关,说明生长调节剂是通过调节插条内代谢物质的含量来促进插穗的生根。
The rooting and physiological biochemistry metabolize changes were studied during rooting of the softwood and plantlets in tissue of cherry＇s stock by treating of growth regulator NAA and IBA with water control. It was showed that the rooting rates and metabolize indexes of the cutting can be improved remarkably during the cutting rooting： When the softwood cutting soaked respectively with 100 mg/L NAA and IBA, the rooting rate 88.3% and 85% of them were obtained. The plantlets could rooting in out-tube after soaking with 100 mg/L NAA, rooting rates in this condition was 86. 1% ; During the primordium formation the soluble sugars, chlorophyll and nucleic acid were increased sharply while the soluble protein were decreased, but it is contrary for the contents of soluble sugar and soluble protein in the course of adventitious root formation, then the soluble sugar gradually increased by assimilated nutriment and leaves photosynthesis in the stage of adventitious root elongation. The results illustrated that the growth regulator promoted rooting by regulating the contents of interior substances of cutting which related with rooting.
出处
《园艺学报》
CAS
CSCD
北大核心
2005年第4期691-694,共4页
Acta Horticulturae Sinica
基金
辽宁省基金项目(2003207008)
关键词
樱桃
生长调节剂
砧木
嫩枝扦插
生根
生化代谢
Cherry
Growth regulator
Stock
Softwood cutting
Rooting
Biochemical metabolize
