摘要
目的观察西乐葆对人胃癌SGC7901细胞凋亡的诱导作用,并探讨其诱导凋亡的机制。方法采用噻唑蓝(MTT)法检测SGC7901细胞的增殖活性,流式细胞仪测定细胞凋亡、线粒体膜电位和胞内游离Ca2+含量。结果SGC7901细胞经25、50、100、200μmol/L西乐葆处理4、8、12、24h后,细胞增殖均明显受到抑制,呈时间和剂量依赖性。50μmol/L西乐葆处理SGC7901细胞4、8、12、24h后,实验组的凋亡率分别为9.2%±0.6%、16.7%±1.6%、20.4%±2.8%和23.9%±2.7%,明显高于对照组的6.8%±0.4%、7.2%±0.3%、7.6%±0.6%和8.3%±0.8%(P<0.05);实验组的线粒体膜电位呈下降的趋势,而胞内游离Ca2+含量则随着时间的延长逐渐增高。结论西乐葆诱导SGC7901细胞凋亡可能涉及到线粒体途径。
To explore the effects of celecoxib, a selective COX-2 inhibitor, on inducing the apoptosis of gastric cancer cells, and to elucidate the concerning mechanisms. Methods Cell viability was measured by MTT assay. Flow cytometry （FCM） was employed to assay apoptosis, rnitochondrial membrane potential and intracellular free Ca^2＋. Results After being exposed to celecoxib （25, 50, 100 and 200μmol/L） for 4, 8, 12 and 24h, the proliferation of SGC-7901 cells was strongly inhibited in a dose-time dependent manner. The apoptosis induced by celecoxib was accompanied with the attenuation of rnitochondrial membrane potential and the elevation of intracellular free Ca^2＋ concentration, suggesting the importance of mitochondria in the apoptotic pathway. Condnsion The mitochondrial pathway may be involved in the apoptosis of gastric cancer SGC-7901 cells induced by celecoxib.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2005年第9期782-784,共3页
Medical Journal of Chinese People's Liberation Army
基金
全军"十五"医药卫生科研基金资助课题(编号01Z075)
关键词
西乐葆
细胞凋亡
线粒体
胃肿瘤
celecoxib
apoptosis
rnitochondria
stomach neoplasms
