摘要
蛋白质芯片技术是采用微阵列方法,对样品中蛋白质进行高通量、高灵敏度分析的技术。它不仅是蛋白质组学研究中强有力的工具,也是临床应用中疾病早期诊断、预后和治疗效果评测的新手段,其研究成果拓展了与人类健康更加贴近的应用领域。本文较为全面地介绍了蛋白质芯片技术的原理和分类、固相基质和蛋白质的固定、信号检测等方面的进展。
Protein microarray has been developed for high throughput and high sensitive experiment, and the strong suits of this technology will make it a promising tool in proteomic research, and clinical field such as early diagnoses, after treatment and the evaluation of treatment. This review introduced the progress of protein microarray technology, including elements and types, solid basis, immobilization of capture molecule, and the detection methods of protein microarray. However, technological challenges in this field are still remaining.
出处
《中国科学基金》
CSCD
北大核心
2005年第5期277-281,共5页
Bulletin of National Natural Science Foundation of China
基金
国家自然科学基金资助项目教育部重点项目博士点基金资助项目
