摘要
目的探讨巨噬细胞炎性蛋白-1α(MIP-1α)、调节激活正常T细胞表达和分泌的细胞因子(RANTES)在呼吸道合胞病毒(RSV)感染发病机制中的作用,探讨这些趋化因子在RSV感染所致哮喘发病机制中的作用。方法收集45例RSV感染患儿血清,其中10例支气管哮喘,15例支气管炎或肺炎,20例上呼吸道感染;无RSV感染的20例健康婴儿作对照。用ELISA法测定其血清MIP—1α及RANTES水平。结果RSV感染患儿血清MIP-1α、RANTES水平明显高于健康婴儿(P<0.05或P<001);支气管哮喘组和支气管炎或肺炎组血清MIP+1α、RANTKS水平明显高于上呼吸道感染组;支气管哮喘组与支气管炎或肺炎组患儿血清MIP—1α、RANTES的水平无显著差异;另外,血清MIP-1α、RANTES水平与血液中嗜酸性粒细胞(Eos)数目密切相关。结论MIF-1α、RANTES参与RSV感染的发病过程,其机制很可能是它们介导Eos、T淋巴细胞、单核细胞的转移,从而导致RSV感染患儿呼吸道Eos聚集并产生炎症,为解释RSV感染后导致哮喘的发生提供了一些理论依据。
Objective To explore the roles of macrophage inflammatory protein- 1α（MIP- 1α） and regulated upon activation normal T cell expressed and secreted（RANTES） in pathogenesis of respiratory syncytial virus（RSV） infection, ,and explore the roles of these chemokines in asthma caused by RSV infection. Methods Serum samples were obtained from 45 infants with RSV infection, in cluding 10 bronchial asthma 15 bronchitis or pneumonia ,20 upper respiratory tract infection ;20 healthy infants with non - RSV infection as the normal group. ELISA method was used to determine the concentrations of MIP- 1α and RANTES in serum. Results MIP - 1α and RANTES levels in infants with RSV infection were much higher than those of non - RSV infected healthy subjects （P〈 0.05 or P〈0.01 ）. The level of bronchial asthma and ,severe RSV infection subjects were much higher than upper respiratory tract infection subjects. But no significant difference between bronchial asthma and svere RSV infection was found. It was also found that serum concentrations of MIP -1α and RANTES were positively correlated with the number of eosinophilis. Conclusions MIP -1α and RANTES are involved in the RSV infection process possibly by recruiting and activating eosinophilis, T lymphocytes and monocytes in the lung inflammatory infiltrate induced by RSV infection. And it may be responsible for the bronchial asthma after RSV infection.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第10期986-988,共3页
Journal of Applied Clinical Pediatrics
