摘要
应用高效液相色谱电化学检测法（ＨＰＬＣ－ＥＣＤ）测定正常及ＭＰＴＰ（１甲基２苯基１，２，３，６四氢吡啶）溃疡大鼠胃体、胃窦和十二指肠粘膜组织中单胺类神经递质，包括肾上腺素（Ｅ）、去甲肾上腺素（ＮＥ）、３，４－双羟苯乙酸（ＤＯＰＡＣ）、多巴胺（ＤＡ）、５羟吲哚乙酸（５ＨＩＡＡ）、高香草酸（ＨＶＡ）和５羟色胺（５ＨＴ）含量的变化。正常大鼠胃体、胃窦和十二指肠粘膜含有丰富的ＤＡ和ＤＯＰＡＣ。侧脑室注射ＭＰＴＰ后大鼠胃体、十二指肠粘膜ＤＡ减少并持续较长时间，减少程度同用药时间、剂量平行。胃体ＤＡ减少的同时伴有５ＨＴ减少、十二指肠５ＨＴ升高。提示ＤＡ在溃疡病的发生机理中具有重要作用，５ＨＴ亦参与溃疡形成。
A high performance liquid chromatography with electrochemical detection(HPLC-ECD)method was employed for determination of monoamines,including epinephrine(E),norepinephrine(NE),3,4 dihydroxyphenylacetic acid(DOPAC),dopamine(DA),5-hydroxy-3-indole acetic acid(5HIAA),homovallilic acid(HVA)and 5-hydroxytryptamine(5HT),in gastric body,antrum and duodenal mucosa of normal rats and rats with MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)induced ulcers.Abundant contents of DA and DOPAC were emerging in normal gastric body,antrum and duodenal mucosa while subsequent reduction of DA concentration were observed in gastric body and duodenal mucosa of rats by MPTP intracerebroventricular injection and persisted for a long time.The degree of reduction was correlated with time and dosage of MPTP administered.of The decrease of 5HT in gastric body and its increase in duodenum were also obsevered at the same time.Such variation of concentrations implied that DA might play an important role in the pathogenesis of ulcerous diseases and 5HT might probably contribute to the development of ulcers.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1996年第3期229-233,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
单胺类
溃疡病
多巴胺
病理
monoamine
ulcer disease
pathogenesis
