摘要
目的调查山东淄博地区汉族人群HLA基因多态性的分布。方法采用序列特异性引物PCR(PCR-SSP)方法,对HLA-A、B、DR位点进行低分辨基因分型;用直接计数法进行抗原频率和基因频率计算。结果得到一组抗原频率和基因频率资料。结论山东淄博地区汉族人群HLA基因具有多态性,PCR-SSP方法进行HLA分型的结果准确可靠。
Objective To investigate the distribution of HLA gene polymorphism of Han people in Zibo area. Methods HLA-A,B,DRB1 alleles were typed at low resolution by PCR-SSP. The antigen frequency and gene frequency were calculated by direct counting. Results A group of data on antigen frequency and gene frequency were obtained. Conclusion The polymorphism is existed on HLA gene distribution of Han population in Zibo area at gene level ,and PCR-SSP is a reliable method for HLA typing.
出处
《临床输血与检验》
CAS
2005年第4期262-264,共3页
Journal of Clinical Transfusion and Laboratory Medicine
