摘要
目的观察奥曲肽对实验性急性坏死性胰腺炎(ANP)大鼠血清TNF-α、IL-6和IL-10的影响,探讨其治疗价值。方法雄性SD大鼠48只,随机平均分为3组:假手术组、ANP组和奥曲肽组。ANP模型以胆胰管逆行注射5%牛磺胆酸钠液(0.1 mg/100 g体重)复制。奥曲肽组为ANP模型建立后30 min经股静脉注射奥曲肽0.9μg/100 g体重。假手术组为胆胰管逆行注射等量生理盐水。观察ANP 诱导术后3 h、6 h血清TNF-α、IL-6、IL-10、血清淀粉酶、腹水量、胰腺大体和组织学的病理改变。结果(1)ANP组3 h、6 h血清淀粉酶明显升高(P<0.01),血清TNF-α、IL-6和IL-10水平也均明显高于假手术组(P<0.001)。(2)奥曲肽组血清淀粉酶、IL-6、IL-10和胰腺组织学评分与ANP组比较无差异,但ANP后3 h、6 h血清TNF-α水平降低(P<0.01),6 h时的腹水量较ANP组减少(P<0.05)。结论奥曲肽降低了ANP时血清TNF-α水平,对急性胰腺炎的治疗可能有益。
Objective To observe changes of serum TNF-α. IL-6 and IL-10 levels in rats with experimental acute necrotic pancreatitis （ANP） treated with Octreotide whereby the therapeutic effects of the drug were evaluated. Methods The acute necrotic pancreatitis （ANP） model was established by injection of 5% sodium taurocholate （0.1 ml/100g） into the common cholangiopancreatic duct in male SD rats. A total of 48 SD rats were equally randomized to three groups： Sham group, ANP group and Octreotide group （30 minutes after inducing the ANP model, the rats received 0.9μg Octreotide per 100 g body weight via the femoral vein. Half of the rats studied were sacrificed at 3 hours and the other half at 6 hours in each group after establishment of the study model. The following parameters were determined： serum amylase, macroscopic pathologic scoring of the lung and pancreas, pancreatic histological pathologic scoring, the amount of ascites and serum levels of TNF-α, IL-6 and IL-10. Results （1）The serum amylase level was significantly increased in ANP groups than that in sham group （P〈 0. 01 ）. The serum levels of TNF-α, IL-6 and IL-10 in ANP groups were significantly higher than those in sham group with P 〈 0. 001, P 〈 0. 001 and P 〈 0. 001 respectively. （2）In Octreotide group, the serum amylase level, both pancreatic and lung macroscopic pathologic scoring and pancreatic histological pathologic scoring showed no improvement. While the amount of ascites at 6 hours decreased （P〈 0.05）. The level of serum TNF-α was significantly decreased （P〈0.01）. There was no change in serum IL-6 and IL-10 levels. Conclusions Octreotide benefits the treatment of acute pancreatitis, possibly by decreasing serum TNF-αlevel.
出处
《胰腺病学》
2005年第3期172-174,共3页
Chinese JOurnal of Pancreatology
