摘要
目的:观察脱氢表雄酮(DHEA)对体外培养的Burk it淋巴瘤(Raji)细胞抗氧化能力的影响。方法:用一定浓度的DHEA作用于体外培养的Raji细胞,同时观察Raji在加用吩嗪硫酸甲酯(PMS)作用前后细胞内葡萄糖-6-磷酸脱氢酶(G-6-PD)、谷光甘肽过氧化物酶(GPX)、谷光甘肽还原酶(GR)活性及还原型谷胱甘肽(GSH)浓度的变化,并与未加DHEA作用的Raji细胞作对照。结果:DHEA浓度≥5.0μmol/L时能明显抑制Raji细胞G-6-PD活性,而对GPX、GR及GSH无明显影响;在氧化压力下(PMS作用)经DHEA处理后Raji细胞G-6-PD、GPX、GR活性无明显升高,细胞内GSH浓度显著降低(P<0.01),并明显低于未经DHEA处理的Raji细胞(P<0.05)。结论:一定浓度的DHEA能抑制Raji细胞G-6-PD活性,并降低其氧化应激能力。
Objective ： To investigate the influence of dehydroepiandrosterone （DHEA） on antioxidant capacity of Raji cell cultured in vitro. Methods： Raji cell line was cultured in vitro in the presence of DHEA and/or phenazine methosulfate（ PMS）, some antioxidant enzymes including glucose-6-phosphate dehydrogenase activity （ G-6-PD ）, glutathione peroxidase activity （ GPX ）, glutathione reductase activity （GR） and intracellular glutathione（GSH） concentration of the DHEA pretreatment cells were evaluated. meanwhile, these results were compared with that from the respective control cells. Results： G-6-PD activities were suppressed significantly and no changes in activities of GPX, GR and intracellular GSH concentration were occurred while Raji cells were cultured in the presence of DHEA at concentration more than 5.0 μmol/L . On the other hand, the DHEA pretreatment cells show no changes in G-6-PD,GPX,GR activities and significantly decreased GSH concentration（P 〈0.01 ）when 50 μmol/L PMS were added in the culture medium compared with those cultured in no PMS added medium. Furthermore, DHEA pretreatment Raji cells showed lower GSH concentration （ P 〈 0.05 ） than no DHEA pretreatment cells in presence of PMS. Conclusion ： DHEA at some concentration can inhibit G-6-PD activity and decrease antioxidant capacity of Raji cell.
出处
《医学研究生学报》
CAS
2005年第9期776-778,783,共4页
Journal of Medical Postgraduates
基金
湖北省自然科学基金资助项目(批准号:2004ABA189)
