摘要
目的探讨PCNA和c-erbB-2在喉良恶性上皮中的表达程度及其与病理分级和预后的关系。方法应用免疫组织化学方法探讨10例单纯性增生、30例异型增生及40例喉鳞状细胞癌的PCNA、c-erbB-2表达。结果单纯性增生PCNA指数为7.0%,轻、中、重度异型增生PCNA指数分别为13.2%、19.4%、26.6%。高、中、低分化鳞癌PCNA指数分别为32.7%、55.4%、78.3%。单纯性增生、异型增生和鳞癌间PCNA指数存在显著的统计学差异(P<0.01)。但高分化鳞癌与重度异型增生间PCNA指数无统计学差异。c-erbB-2在单纯性增生及异型增生中均无表达,在鳞癌中有表达,但在高、中、低分化鳞癌间无显著差异。对40例喉鳞癌随访5年,有16例死亡,其中13例PCNA指数>78%,即PCNA指数越高,分化越差,预后越差。结论PCNA具有较好的客观性评价异型增生的程度和鳞癌分化程度,与患者预后密切相关。c-erbB-2不能作为异型增生和癌的分级的指标。
Objective To study the expression of proliferating cell nuclear antigen （PCNA） and c-erbB-2 in benign and malignant epithelium of the larynx and to evaluate the relationship of PCNA and c-erbB-2 with the pathologic grade and prognosis. Methods The expression of PCNA and c-erbB-2 was detected in 10 cases of simple hyperplasia, 30 cases of dysplasia and 40 cases of squamous carcinoma by immunohistochemical staining. Results The PCNA index was 7.0% in simple hyperplasia and 13.2%, 19.4% and 26.6% in light, moderate and severe dysplasia respectively, and 32.7%, 55.4% and 78.3% in high, moderate and low differentiation squamous carcinoma, with significant statistic difference in between simple hyperplasia, dysplasia and carcinoma except in between high differentiated squamous carcinoma and severe dysplasia. The expression of c-erbB-2 was not found in simple hyperplasia but in squamous carcinoma without significant difference in between high, moderate and low differentiated carcinoma. Following up for five years, 16 of 40 patients died, among which 13 cases were low differentiated carcinoma. Conclusions PCNA may be a more objective means to evaluate the grades of dysplasia and carcinoma but not c- erbB-2.
出处
《解剖科学进展》
CAS
2005年第3期237-239,共3页
Progress of Anatomical Sciences
基金
留学回国人员科研启动基金资助项目([2002]247号)
