摘要
目的了解血培养分离菌的菌种分布及耐药情况。方法用 BACTEC～9120全自动血培养系统对血培养标本进行检测。阳性的标本进行常规细菌鉴定,药敏试验采用美国临床实验室标准委员会(NCCLS)推荐的标准纸片扩散法。结果 3697份血培养标本,分离出病原菌409株,其中革兰阴性菌252株(61.6%),革兰阳性菌108株(26.4%),真菌49株(12.0%)。革兰阴性菌以大肠杆菌检出率最高,革兰阳性菌以金黄色葡萄球菌检出率最高,真菌感染以白色念珠菌略多。大肠杆菌、肺炎克雷伯菌中产超广谱β-内酰胺酶(ESBLs)菌株分别占42.2%、29.6%,金黄色葡萄球菌、凝固酶阴性葡萄球菌耐甲氧西林菌株分别占34.3%、72.2%。结论血培养分离菌菌种分布广泛,且耐药菌株检出率相当高,因此加强血培养检测和合理应用抗菌药物显得尤为重要。
Objective To mvestigate the distribution and drug resistant of the microorganism which were cultivated out of blood speci- mens.Method By using BACTEC-9120 automicrobic system the clinical blood specimens collected from patients were detected Identified the species of the bacteria and Antibiogram for them were done by a routine agar disk diffusion assay with Mueller-Hinton agar plates and antibiotic -containing disks which was recommended by NCCLS.Result 409 bacteria were detected out of 3697 clinical blood,there were 252 gram-neg- ative bacteria(61.6%),108 gram-positive bacteria(26.4%)and 49 fungi(12.0%).Compare to the other gram-negative bacteria,Es- cherichia coil was detected by the highest percentage,for the gram-positive bacteria,it is Staphylococcus aureus that has the highest detectrate, and for the fungi,it is a little bit more Chanee for Candida albicans.ESBLs produced in Escherichia coil and Klebsiella pneumoniae account for 42.2% and 29.6% respectively.The strain of methcillin-resistant Staphylococcus aureus and Coagulase negative Staphylococci account for 34.3% and 72.2%.conclusion The bacteria cultivated from blood specimen had a widely distribution and the percentage of bacteria which were antibiotics-resistant was high,so it's especially important to emphasis the detections of blood specimens and to use antibiotics reasonably.
出处
《医学研究通讯》
2005年第9期46-48,共3页
Bulletin of Medical Research
