摘要
目的初步探讨江苏省结核分支杆菌的数目可变串联重复序列(VNTR)的分布特征。方法采用多位点串联重复序列(MLVA)分型方法,对从江苏省病人分离的结核分支杆菌的VNTR进行PCR扩增,2%琼脂糖凝胶电泳,通过BioNumerics3.0软件分析其VNTR的多态性和聚类分析。结果共选取了15个VNTR位点,对67株结核分支杆菌进行了检测分析,结果显示明显的基因多态性,可分8个基因型(Ⅰ～Ⅶ型),其中较多的一型菌株占32.6%,其他型别菌株数量较接近,没有特别明显的优势型别。结论江苏省结核分支杆菌具有明显的多态性,MLVA分型技术具有稳定、简单、可重复的优点,可用于结核分支杆菌的流行病学调查。
To investigate the distribution pattern of the variable number tandem repeats （VNTR） in Mycobacterium tuberculosis isolated from Jiangsu province, the VNTR from strains of M. tb isolaterd in these area was amplified by PCR and was typed by means of methods for multiple loci tandem repeats typing. The polymorphism and clustering were analyzed by PCR, elctrophoresis on 2% agarose and the BioNumberies （version 3.0） software. It was found that the distribution of VNTR from 67 strains of M. tb. showed definite polymorphism, and 8 different genotypes were finally demonstrated in which 32.6 % of strains were dustered to one genotype, and the others were scattered. It is concluded that the strains of M. tb isolated in Jiangsu province show definite polymorphism and the method using the multiple loci tandem repeats typing appears to be simple, stable and reproducible and can be used for the epidemiological survey of tuberculosis.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2005年第9期755-757,共3页
Chinese Journal of Zoonoses
基金
江苏省卫生厅科研基金(H200335)
国家"十五"重点项目(2001DEA10007)。
