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荧光光度法直接测定盐酸异丙肾上腺素的含量 被引量:7

Direct Determination of Isoprenalinum by Fluorescence Spectrophotometry
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摘要 盐酸异丙肾上腺素在伯瑞坦-罗比森缓冲溶液(pH=6.80)介质中有很强的荧光。其最大激发波长和发射波长为287nm和622nm。荧光强度与纯盐酸异丙肾上腺素浓度在0.1—2.3mg/L范围内呈线性关系。回归方程F=351.91C(mg/L)+139.02,r=0.9995,检出限为0.0087mg/L。测定注射用针剂中盐酸异丙肾上腺素的含量,方法简单,快捷,灵敏度高,结果令人满意。 In the medium of buffer solution (pH=6.80), isoprenalinum has strong characteristics at excitation wavelength λex=287nm and emission wavelength λem=622nm. The linear range of concentration of isoprenalinum with intensity is of 0.1-2.3mg/L with the equation of F=351.91C (mg/L)+139.02, r= 0.9995. The detection limit is 0.0087mg/L. The method is easy, repaid, sensitive and was applied to the determination of isoprenalinum in medicament with satisfactory results.
作者 乔善宝
出处 《光谱实验室》 CAS CSCD 2005年第4期737-739,共3页 Chinese Journal of Spectroscopy Laboratory
基金 江苏省教育厅科研基金(03KJD150241)资助项目
关键词 盐酸异丙肾上腺素 荧光光度法 Isoprenalinum, Fluorescence Spectrophotometry
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