摘要
目的建立应用小动物呼吸机实施小动物吸入麻醉的方法,并研究其体外模式的动态过程。方法将麻醉机的气体输出口与小动物呼吸机进气口相连,呼吸机回路与模拟动物肺的注射器连接。按照兔、大鼠和小鼠三种体外模型设定呼吸机呼吸参数,分别测定呼吸机进气口处和注射器呼气末气体麻醉药浓度,计算吸入阶段注射器呼气末气体麻醉药浓度升高百分率(CE/CI)和消除阶段注射器呼气末气体浓度降低百分率(C'E/C0)。结果三种体外模型吸入阶段CE/CI逐渐升高,CE/CI与时间的倒数成线性关系,相关系数分别为0.90、0.95和0.93(P<0.01);清除阶段C'E/C0逐渐降低,C'E/C0与时间的倒数成线性关系,相关系数分别为0.90、0.94和0.95(P<0.01)。结论将小动物呼吸机与麻醉机联用能完成小动物吸入麻醉,建立了应用小动物呼吸机实施小动物吸入麻醉的体外模式,为今后进行吸入麻醉药在小动物的药理学研究建立了可靠的体外模型。
Objective To set up a method of applying inhalation anesthesia in rodent using rodent ventilator and to study the dynamic procedure of the in vitro model. Methods The output port of the anesthesia machine was connected to the input port of the rodent ventilator,which was connected to a syringe simulating the lung. After supply of anesthetic gas, the gas samples from the input port of the ventilator and the syringe in the end-expiratory phase were collected at 10,20,30,40,50,, 60,90,120,180,300,600 and 900 seconds respectively and were determined using the gas chromatography（GC）. The ratios of the anesthetic concentrations of the syringe to that of the input port were calculated （CE/CI）. In elimination phase, the gas samples from the syringe were collected at 0,10,20,30,40,50,60,90,120,180,300,600 and 900 seconds respectively and were determined by GC. The ratios of the anesthetic concentrations of the gas at 10,20,30,40,50,60,90,120,180,300,600 and 900 seconds to that at 0 second were calculated（C＇E/C0）. Results CE/CI increased in the inhalation phase, there was an inverse relationship between CE/CI and time, the correlation coefficients were 0. 90,0. 95 and 0. 93 respectively （P〈0. 01）. The mathematical fitting equations were y=-0. 19＋0. 19x^-1,y=-7.75＋0. 99^x-1,and y=-7.21＋0. 97x^-1 respectively. C＇E/C0 decreased in the elimination phase,the correlation coefficients were 0. 90,0. 94 and 0. 95 respectively（P〈0. 01）. The mathematic fitting eqations were y=5.65-0. 02^x-1 ,y=7.82-0. 01^x-1 ,and y=-8. 20-0. 01x^-1, respectively. Conclusion The in vitro model of rodent inhalation anesthesia using the rodent ventilator was set up. The establishment of this model has provided a basis for studies on inhalation anesthesia in rodents.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期727-729,742,共4页
Journal of Sichuan University(Medical Sciences)
关键词
动物呼吸机
麻醉机
异氟醚
Rodent ventilator
Anesthesia machine
Isoflurane
