摘要
目的研究经亲水性交联剂乙二醇缩水甘油醚(EX-810)处理的猪胸腔动脉的性能特点,为构建组织工程化血管提供理想的支架材料。方法用40ml/L的EX-810处理猪胸腔动脉7min^72h,同时以6.25g/L的戊二醛处理血管作对照。所获材料分别进行外观、光镜与扫描电镜检查;另外还进行交联指数、氨基酸成分及力学性能的测定。结果EX-810既能有效去除和降低组织中产生抗原性的细胞成分和自由氨基,又保持了血管总体组织构架的完整;同时经它处理的血管组织比戊二醛处理者有更好的柔韧性、更接近天然血管组织,在各项力学性能方面也与天然血管组织极为相似。结论EX-810处理的猪胸腔动脉在所检测的各个方面,都与天然的新鲜血管相似,其自身又具有易于保存、低细胞毒性及低抗原性等优点,是作为组织工程化血管支架的理想生物材料。
Objective To investigate the characteristics of porcine thoracic arteries fixed with ethylene glycol diglycidyl ether （EX-810） and to provide the proper scaffold materials for tissue-engineered blood vessel. Methods The porcine thoracic arteries were respectively treated with 40 ml/L EX-810 and 6. 25 g/L glutaraldehyde, and then they were examined with naked-eye, light microscope and scanning electron microscope. The fixation index determination, the amino acid analysis and the biomechanics test were also performed. Results The antigenicity of vascular tissues can be diminished by EX-810 through getting rid of cell in the vascular tissues or reducing the level of free amino groups in the vascular tissues. The structural integrity of vascular tissues can be preserved after treatment with EX-810. It was also found that the EX-810-fixed porcine vascular tissues appeared more similar to the natural vascular tissues in color and mechanical properties, and were more pliable than the glutaraldehyde-fixed tissues. Conclusion The EX-810-fixed porcine thoracic arteries with low cytotoxicity and low antigenicity showed favorable characteristic similar to those of natural vessel, and it should be a promising material for fabricating scaffold of tissue-engineered blood vessel.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期692-695,共4页
Journal of Sichuan University(Medical Sciences)
关键词
乙二醇缩水甘油醚
猪胸腔动脉
交联
力学性能
Ethylene glycol diglycidyl ether
Porcine thoracic arteries
Crosslinking
Mechanical properties
