摘要
目的探讨水通道蛋白4(AQP4)在原代培养新生大鼠星形胶质细胞(AC)缺氧和缺氧后再复氧损伤模型中的表达改变及其意义。方法原代培养成熟的AC随机分成常氧培养组(对照组)、缺氧组和复氧组。缺氧组AC在缺氧条件下分别培养3h、6h、12h和24h,复氧组AC缺氧培养12h后更换为常氧培养,分别培养3h、6h、12h和24h。各组样品采用RealTimePCR和免疫荧光染色分别测定AQP4mRNA和蛋白表达水平。结果随着缺氧时间延长,AQP4mRNA和蛋白表达水平进行性降低,复氧时则先降低,后回升。结论AQP4表达下降可能与脑水肿的发生有关,AQP4可能对脑内渗透平衡重建起重要作用。
Objective To investigate the expression of aquaorin-4 （AQP4） in the cultured neonatal rat primary astrocytes during hypoxia and during reoxygenation after hypoxia. Methods The astrocytes were randomly assigned to the hypoxia group （the astrocytes were exposed to hypoxia for 3 h, 6 h, 12 h and 24 h respectively）, the reoxygenation group （after exposure to 12 h hypoxia, the astrocytes were returned to normoxic cultures for 3 h, 6 h, 12 h and 24 h respectively）, and the normoxic group （control group）. AQP4 mRNA expression in astrocytes of all groups was detected by Real Time PCR, and AQP4 protein expression was determined by immunofluorescence. Results The AQP4 mRNA and protein expressions significantly decreased with the time of hypoxia, and during reoxygenation, the decreased expressions were gradually up-reguated. Conclusion It is likely that the decreased expression of AQP4 is associated with brain edema and in such brain edema conditions AQP4 may play a prominent role in the reestablishment of brain osmotic equilibrium.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期641-644,共4页
Journal of Sichuan University(Medical Sciences)
