摘要
目的探讨新生大鼠缺氧缺血性脑损伤（hypoxic—ischemic brain damage，HIBD）后脑组织水通道蛋白（AQP4）基因与蛋白表达及碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）对HIBD治疗效果的影响。方法生后7d新生大鼠80只，随机分为正常对照组10只，HIBD组30只，bFGF治疗组（4μ／kg，腹腔注射，连续15d）30只，生理盐水治疗组10只。采用RT—PCR和蛋白印迹技术检测脑缺氧缺血后及经bFGF治疗不同时间段AQP4mRNA基因与蛋白的表达。结果HIBD后5h，脑组织AQP4mRNA表达增加；HIBD后7d达到高峰（1．93±0．11），15d后仍高于正常组织；HIBD后5h、3d．7d、15d、17d有不同程度的AQP4蛋白表达，3d、7d最为明显，而正常对照组未见蛋白表达。HIBE）后腹腔注射bFGF，3d后脑组织AQP4mRNA有明显变化（0．79±0．15），15d明显降低到（0．47±0．06）；第7d蛋白表达逐渐减弱，15d、17d蛋白不表达。结论AQP4与HIBD的形成密切相关；bFGF对HIBD有明显的改善作用，可能与其保护作用有关。
Objective To study the expression of AQP4 in the hindbrain of neonatal rats with hypoxic-ischemic brain damage （HIBD） and to explore the effect of basic fibroblast growth factor （bFGF） on the treatment of HIBD. Methods Eighty neonatal rats aged 7-day were divided into randomly-control group （ 10 rats）, HIBD group （30 rats）, bFGF treated group （30 rats）, and NS treated group （10 rats）. HIBD were established by exposing rats to 92% N2 mixed with （8 ± 1） % O2 for 20 minutes after ligating the left carotis d the rats. bFGF treatel group were given bFGF 4u/kg for 15 days via intraperitoneal injection. The expression of AQP4 mRNA and AQP4 protein of neonatal rats with HIBD were examined with RT-PCR and Western blotting at different stage after hypoxia. Results In HIBD group, the expression of AQP4 mRNA in hindbrain was higher than that in control group at 5 h after HIBD. reached peak at 7 d （ 1.93 ± 0.11 ） after hypoxia, and remained at a high level until 15 d （ 1.41 ± 0.23） after hypoxia. In bFGF group, the expression of AQP4 mRNA in hindbrain was obviously changed at 3 d （0.79 ± 0.15） after hypoxia, decreased evidently at 15 d （0.47 ±0.06）. In bFGF group, the expressions of AQP4 protein at 5 h, 3 d. 7 d after HIBD were positive at different degree, with the decreasing level at 7 d and no protein at 15 d and 17 d. Meanwhile, the expressions in normal control group were negative. Conclusion AQP4 was closely related with the formation of HIBD. After treating with bFGF HIBD can improved significantly, which may be due to the protection role of bFGF.
出处
《中国小儿急救医学》
CAS
2008年第1期58-60,共3页
Chinese Pediatric Emergency Medicine
