摘要
采用质谱、圆二色谱、荧光光谱与细胞周期分析技术研究了重组内皮抑素的蛋白结构和对内皮细胞的作用机制.研究发现,以包涵体方式表达的蛋白中会有未被降解的N末端甲硫氨酸产物,重组内皮抑素在G2期阻断内皮细胞的生长.表明内皮抑素引发内皮细胞凋亡与细胞周期相关,为抗血管生成治疗肿瘤研究奠定了实验基础.
The structure of recombinant endostatin and mechanism of acting on endothelial cell were studied by mass spectrometry, circular dichroism spectroscopy, fluorescence spectroscopy and cell cycle analysis technique. It was found that the recombinant protein prepared from inclusion body of E. coli may combinate the derivated protein with N-terminal Met. The mechanism by which recombinant endostatin causes G_2 arrest in endothelial cells was observed, which demonstrated that the apoptosis of endothelial cell induced by endostatin was correlated with cell cycle. The results lay the foundation for the antiangiogenesis therapy of the tumor.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2005年第5期870-873,F006,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金重大项目(批准号:29890280)资助.
关键词
重组内皮抑素
质谱
圆二色谱
细胞周期
Recombinant endostatin
Mass spectrometry
Circular dichroism spectroscopy
Cell cycle
