摘要
首次利用人隐静脉内皮细胞(HSVECs)在体外建立血管生成模型,并与脐静脉内皮细胞(HUVECs)模型进行比较.在不加入任何生长因子的条件下,在三维胶原系统中经过3 d的培养,HSVECs能形成毛细血管样网状结构.genistein是一种酪氨酸酶抑制剂,它能影响毛细血管网的形成.与HUVECs模型比较两者存在明显的差异性.HSVECs在体外三维模型中能形成一种变异型的毛细血管样网状结构.
HSVECs angiogenesis model was first developed and compared with HUVECs angiogenesis model. HSVECs and HUVECs were separately sandwiched into the 3-D collagen gels to culture the angiogenesis model in vitro; the anti-angiogenic effects of genistein was assessed in this system. HSVECs have the capacity to form capillary-like structures in this model, the organization process was slower than HUVECs angiogenesis model. This new HSVEC angiogenesis model has been proved to be as a anti-angiogenic screening model.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第5期457-460,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南大学理(工)科校级科研项目资助(K1059069)
关键词
内皮细胞
血管生成
体外模型
HSVECs
angiogenesis model
capillary-like tubes
