摘要
采用激光扫描共聚焦显微镜观察受不同参数高功率微波辐照后的大鼠精子细胞内Ca2+的变化,同时观察了辐照后大鼠的生殖细胞微核率、畸形率、精子数量、存活率和顶体酶反应的变化。实验表明:此条件下的高功率微波辐照对雄性大鼠的生殖细胞微核率和畸形率没有明显的损伤效应,但4×105次脉冲的高功率微波辐照对大鼠的顶体酶反应有较明显的抑制作用,可能通过改变细胞内第二信使的Ca2+浓度来影响其生殖细胞功能。
The effect of HPM on the intracellular calcium of sperm cells was determined using laser scanning confocal microscopy. The abnormality, micronucleus frequency, livability, numbers and the acrosin reaction of the sperm cells were studied at the same time. The experiments indicate that there are no visible damaging effects on the abnormality and micronucleus frequency of the sperm cells after HPM irradiation,but the acrosin reaction of the rat sperm cells was restrained significantly after 4 × 10^5 pulses irradiation. The function of the sperm cells was probably induced by changing the intracytoplasmic calcium concentration of the rat sperm cells.
出处
《强激光与粒子束》
EI
CAS
CSCD
北大核心
2005年第8期1267-1271,共5页
High Power Laser and Particle Beams
基金
国家自然科学基金资助课题(60471032)
国家863计划项目资助课题
