摘要
目的:研究中国人群中白细胞介素-1(IL-1)基因多态性与侵袭性牙周炎(AgP)之间的关系。方法:提取122例AgP患者和95例健康对照者外周静脉血基因组DNA,采用聚合酶链反应(PCR)分析IL-1A+4845、IL-1B+3954位点的单核苷酸多态性(SNP)及IL-1RN第二内含子中的可变数目重复序列(VNTR)多态性,采用多因素Logistic回归模型,进行两组之间基因型、等位基因分布差异的比较。结果:在IL-1A+4845位点,AgP组男性患者A2+基因型的频率较男性健康组显著升高(P<0.05=0.039),等位基因A2的携带率也显著升高(P<0.05=0.049);未发现IL-1B+3954位点和IL-1RNVNTR的多态性与AgP关联;未发现IL-1各复合基因型与AgP存在明显关联性。结论:IL-1A+4845位点的SNP可能与中国人群中男性个体的AgP易感性有关。
PURPOSE： To study whether interleukin-1 （IL-1） genotypes and/or alleles were associated with aggressive periodontitis （AgP）. METHODS： Anti-coagulated peripheral blood samples were obtained from 122 AgP patients and 95 healthy controls. Genomic DNA was extracted from each sample. Single nucleotide polymorphisms （SNPs） at IL-1A ＋4845 and IL-1B ＋3954 were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism （PCRRFLP） assay. The polymorphism of a variable number tandem repeat （VNTR） in intron 2 of IL-1RN was detected by PCR amplification and fragment size analysis. The genotypes and/or alleles distribution differences between two groups were analyzed by logistic regression analysis. RESULTS： The frequencies of A2^＋ genotype and allele 2 at IL-1A ＋4845 were significantly increased in male AgP patients compared with male controls （genotype： 18.0% vs 4.3%, adjusted OR=5.58, 95% CI=1.09-28.68,P=0.039;allele： 9.0% vs 2.2%, adjusted OR=4.97, 95% CI=1.01-24.50, P=0.049）. CONCLUSIONS： The IL-1A ＋4845 polymorphism may be associated with AgP susceptibility in Chinese males. Supported by National Natural Science Foundation of China （Grant No.30271411）.
出处
《上海口腔医学》
CAS
CSCD
2005年第4期333-337,共5页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30271411)
教育部教育振兴行动计划特殊专项("九八五"工程)(北京大学人类疾病基因研究中心2001-8)
国家高科技项目(863计划)(2002AA217091)
关键词
牙周炎
多态性
白细胞介素1
易感性
Periodontitis
Polymorphism
Interleukin-1
Susceptibility
