摘要
目的:观察侧脑室穿刺对豚鼠听觉脑干诱发电位(ABR)及内耳病理形态的影响.方法:健康成年豚鼠随机分为手术对照组(只暴露硬脑膜)、侧脑室穿刺Ⅰ组(暴露硬脑膜,右侧脑室穿刺,收集流出的脑脊液约0.1ml)、穿刺Ⅱ组(将穿刺流出的脑脊液即刻缓慢注入侧脑室)、穿刺Ⅲ组(向侧脑室中缓慢注入0.1 ml生理盐水),每组10只动物.分别于术前、术后1 h、24 h行ABR测试,记录各组豚鼠双耳听力阈值及70 dB时各波潜伏期和Ⅰ～Ⅲ波间期,取耳蜗组织做病理检查.以不施加任何处理因素的6只豚鼠为正常对照.结果:术前各组豚鼠ABR各波潜伏期、Ⅰ～Ⅲ波间期及阈值差异均无统计学意义(P＞0.05);术后1 h,侧脑室穿刺Ⅰ组ABR各波潜伏期、Ⅰ～Ⅲ波间期较术前延长,阈值升高,术后24 h各指标恢复正常;其余各组动物上述各指标术前、术后1 h、24 h差异均无统计学意义(P＞0.05).手术对照组动物术后1 h和24 h耳蜗病理切片未见异常.侧脑室穿刺组动物术后1 h时,耳蜗底转鼓阶出现红细胞沉积现象;24 h后各阶和前庭阶中均可有红细胞出现.结论:一定量脑脊液丧失会引起豚鼠短暂、可逆性听力下降,而脑脊液体积或压力适量增加对豚鼠ABR无明显影响.侧脑室穿刺后,脑脊液中红细胞可经耳蜗导管到达外淋巴,并随外淋巴纵行流动广泛分布到各转鼓阶和前庭阶.
Aim ： To study the changes of auditory brainstem response （ABR） and morphology of the inner ear in guinea pigs after lateral ventricle puncture. Methods： A total of 40 healthy guinea pigs were randomly and equally allocated into 4 groups： the operative control group（ only exposed dura） , the lateral ventricle puncture group Ⅰ （exposed dura and collected about 0. 1 ml CSF by lateral ventricle puncture） ,the puncture group Ⅱ （ collected CSF and injected it back into the lateral ventricle immediately and slowly） ,the puncture group Ⅲ （ injected 0.1 ml NS into the lateral ventricle slowly）. ABR was measured before operation, 1 h and 24 h after operation. The latency of each wave, inter-peak period at 70 dB and threshold value were recorded. The cochlear specimens were stained in HE. Six guinea pigs without any treatment was used as normal control. Results： At 1 h after operation, the latency of each wave and Ⅰ - Ⅲ inter-peak periods in the lateral ventricle puncture group Ⅰ were prolonged and the threshold values increased significantly, which returned to normal at 24 h after operation. The above parameters had no significant changes in other groups（ P 〉0.05 ）. Red blood cell deposition was observed in the perilymphatic space of the cochlea at 1 h afere operation in the lateral ventricle puncture groups, and at 24 h, red blood cells distributed in all scalae tympani. Conclusion ： Loss of cerebrospinal fluid in a certain extent can result in transitory and reversible hearing loss, while ABR had no significant change after the same amount of NS being injected into CSF. Red blood cells in CSF can reach perilymphatic space through the cochlear canal, and widely distribute in scalae tympani and scala vestibuli after lateral ventricle puncture.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第5期863-865,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
脑脊液
迷路液
听觉脑干诱发电位
豚鼠
内耳
cerebrospinal fluid
labyrinthine fluid
auditory brainstem response
guinea pig
inner ear
