摘要
目的对严重急性呼吸综合征(SARS)患者脾淋巴细胞进行定量分析,为SARS的病理变化和发病机制的探讨提供证据。方法通过免疫组织化学技术对6例SARS死亡患者脾和6例意外死亡者正常脾CD3+、CD4+、CD8+T细胞及CD20+B细胞的分布进行观察,并进行图像分析。结果SARS患者脾内的脾小体及动脉周围淋巴鞘(白髓)均遭到严重损害。动脉周围淋巴鞘的数量减少90·39%;脾小体减少80%左右,有的甚至完全消失;脾红髓广泛出血坏死。红髓内的CD3+T细胞平均数较正常减少71·76%,有的甚至完全消失,CD4+T细胞和CD8+T细胞分别平均减少86%和84%。CD20+B细胞减少80%以上。结论定量分析显示,SARS死亡患者脾中T细胞和B细胞普遍严重减少,提示SARS患者免疫系统遭到严重破坏,并可能是疾病的原发性损伤。
Objective To investigate the distribution and the number of splenic lymphocytes in the patients with severe acute respiratory syndrome（SRAS）. Methods Immunohistochemical method with four specific antibodles（CD3, CIM, CD8, CD20） was used to detect the distribution of lymphocyte special antigens in the spleens of six cases who died of SARS and six normal cases as the controls. The numbers of lymphocytes were analyzed with image analysis system. Results In the spleen of SARS patients, splenic corpuscle and perlarterial lymphatic sheath （white pulp） were damaged severely. In the number of periarterial lymphatic sheath decreased 93.39% and in the number of splenic capsule decreased about 80% even disappeared at all. Red pulp hemorrhage necrosis was widely spread. In red pulp the number of CD3^＋ T cells decreased 71.76%, even disappeared at all, in the number of CIM^＋ and CD8^＋ T cells markedly decreased 86% and 84% respectively. The number of CD20^＋ B cell decreased more than 80%. Conclusion T cells and B cells in spleens of SARS patients decreased widely spread and markedly in number, indicating that the immune system had been damaged severely, and the destroyment of the immunosystem may be the primary lesion of SARS.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第4期417-421,共5页
Acta Anatomica Sinica
基金
北京大学和中国科学院SARS研究专项资助项目(2003AA208107)
关键词
严重急性呼吸综合征
T细胞
B细胞
免疫组织化学定量分析
脾
Severe acute respiratory syndrome（SARS）
T cells
B cells
lmmunohistochemical quantity analysis
Spleen
