摘要
【目的】研究沙眼衣原体阴道内感染引起小鼠输卵管炎症的机理、病理改变。【方法】通过雌性小鼠阴道内接种小鼠肺炎(MoPn)沙眼衣原体,感染后不同时间观察小鼠输卵管病变情况,并通过光镜、免疫组化和电镜研究其病理改变。【结果】感染早期小鼠输卵管出现急性炎症改变,病理表现为黏膜层水肿、脱落和坏死;电镜下,在黏膜上皮细胞中找到沙眼衣原体。感染后期小鼠输卵管出现输卵管阻塞和积水,病理表现为黏膜皱襞减少,黏膜层纤毛柱状上皮细胞成矮柱状,顶部纤毛消失,输卵管壁增厚、纤维组织增生和淋巴细胞浸润,免疫组化显示以CD4+T细胞浸润为主。【结论】阴道内沙眼衣原体感染可逆行感染引起输卵管炎症、粘连、阻塞和积水;其病理基础早期为黏膜层急性炎症改变和后期为输卵管壁增厚、纤维组织增生;局部以Th1细胞介导为主的细胞免疫,导致了慢性输卵管炎的病理改变。
[Objective] To investigate the pathogenesis of salpingitis in mice by intravaginal inoculation of Chlamydia trachomatis, and observe the histopathologic changes of oviduct after infection. [Methods] Female mice were infected by the Chlamydia trachomatis agent of mouse pneumonitis （MoPn） by intravaginal inoculation. The anatomical changes of oviduct were observed, then the histopathological changes of oviduct were examined by light microscopy, immunohistochemical staining, and electronic microscopy in different phases after infection. [Result] An acute inflammation was observed in the early stage of postinoculation. The oviduct mucosa was manifested by edema, erosion, and necrosis. Under electronic microscope, agents of MoPn were easily found in the columnar epithelium of the mucosa. After that, a chronic inflammation occurred and the oviduct showed occluded and hydrosalpinx. Pathologic findings were characterized by decreased mucosa plica, short columnar epithelial cell, disappeared cell cilium, thick wall, fibrosis, and infiltrated lymphocyte. Immunohistochemical staining showed mainly a great number of CD4^＋ T cells. [Conclusion] Intravaginal inoculation with Chlamydia trachomatis MoPn can induce salpingitis, result in conglutination occlusion and hydrosalpinx, and these changes are based on the acute mucosa inflammation and the chronic pathological change of thicken and fibrosis of oviduct. This study shows the importance of Th1 cell-mediated immunity in the chronic oviduct inflammation.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第5期520-522,532,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划项目(2004B34201011)
关键词
沙眼衣原体
动物模型
输卵管炎
Chlamydia trachomatis
animal model
salpingitis
