摘要
将猪细小病毒南京毒株1(N J-1)、南京毒株2(N J-2)和7909毒株接种于PK-15细胞,用免疫荧光检测方法研究了猪细小病毒增殖的基本特性与规律。在PK-15细胞中,3个毒株的增殖规律基本相似,均在感染后12 h即可检测到荧光,表明其子代病毒粒子产生,随后逐渐增多,在感染后48 h荧光几乎遍布所有细胞,随后荧光开始衰减,在84 h时病毒引起细胞大面积崩解,荧光呈现岛屿状分布。通过绘制其一步法生长曲线可知,在病毒感染后12 h,细胞培养液中的病毒TC ID50/mL为2.0左右,随后逐渐增高,感染后60 h 3种毒株的TC ID50/mL均达到最高,子代病毒粒子向细胞外释放也达到高峰,其后TC ID50逐渐下降。培养液中病毒粒子的半寿期为7 h。
Some multiplication properties of three strains of porcine parvovirus （PPV） in porcine kidney cells were studied by using the immunofluorescence technique. The results indicated that the three strains have the similar characteristics of the multiplication. The virus was observed within cytoplasm at 12 h postinoculation,followed by the highest product at 48 h after inoculation. Then the viral particles inside host cells began to reduce and caused host cells to crack,releasing from cells. Based on the results,the one-step growth curve of cells was drawn. It was showed that the virus escaped from host cells at 12 h after infection. The viral titers were reached to the highest level at 60 h postinoculation and the viral concentration declined from then on.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2005年第5期453-455,共3页
Chinese Journal of Veterinary Science
基金
河南省重大科技攻关项目(0223013800)
关键词
猪细小病毒
PK-15细胞
增殖
免疫荧光试验
porcine parvovirus （PPV）
PK- 15 cell
replication
immunofluorescence technique （FITC）
