摘要
采用反相高效液相色谱法,在两根串联的C18柱上以甲醇-水为流动相,在8min内将尿素和氨基甲酸甲酯进行分离,在215 nm波长下检测.在甲醇溶液中的质量浓度0～16%和0～50%范围内其峰面积和质量浓度呈良好的线性关系,线性回归方程分别为AUrea=148565x-39384,R2=0.9995;AMC=69055x90493,R2=0.9985.
Separation and determination of urea and methyl carbamate by reversed phase high performance liquid chromatography was performed within 8 minutes on a dual calunm system, using methanol-water as a mobile phase at a wavelength of 215 nm. A linear relationship between mass concentration and peak area was obtained over the range 0- 16% with a correlation coefficient of 0.9995 for urea and 0 -50% with a correlation coefficient of 0.9985 for methyl carbamate.
出处
《分析试验室》
CAS
CSCD
北大核心
2005年第8期85-87,共3页
Chinese Journal of Analysis Laboratory
基金
浙江省科技厅(2004C31053)
浙江丰登化工股份有限公司资助
关键词
反相高效液相色谱
尿素
氨基甲酸甲酯
Reversed phase high performance liquid chromatography
Urea
Methyl carbamate
