摘要
提出了借对乙酰基偶氮氯膦(CPApA)-钡(Ⅱ)-乳化剂OP与蛋白质显色反应分光光度法测定蛋白质的高灵敏方法.在pH 2.5Britton-Robinson缓冲介质中,蛋白质可与CPApA-钡(Ⅱ)-乳化剂OP形成蓝色复合物,蛋白质的加入可使Ba(Ⅱ)-CPApA配合物溶液增色且增色程度与蛋白质的质量浓度成正比.体系的最大吸收波长位于362 nm,测定白蛋白(BSA)表观摩尔吸光系数ε362nm=6.11×103L·moL-1·cm-1,BSA在0～20 mg/L浓度范围内遵守比耳定律.加入乙醇可提高体系的稳定性和灵敏度.表面活性剂OP的加入,显著地提高体系的稳定性.血清中常见组分不干扰蛋白质的测定.所提出的方法可用于人血清样品中蛋白质总量的测定.
A highly sensitive spectrophotometric method is proposed for the determination of protein with p-acetylchlorophosphonazo-barium （ Ⅱ ）-emulgent OP-protein system. In a Britton-Robinson buffer medium of pH 2.5, the bovine serum albumin （BSA） can form a blue complex with Ba（ Ⅱ ）-CPApA complex. The addition of protein can enhance the colour of the complex and the degree of increase colour is proportional to the concerntration of the protein. The maximum absorption wavelength of the system is at 362 nm. The apparent molar absorptivity for the determination of BSA is 6.11 ×10^5L·moL^-1·cm^-1 Beer＇s law is obeyed over the range of 0 - 20 mg/L for BSA. The addition of ethanol can enhance the stability and sensitivity of the system. The addition of surfactant OP can obviously enhance the stability of the system. The common components in serum do not interfere with the determination of protein-, The proposed method was used in the determination of the total protein in human serum samples with satisfactory results. The method possesses the advantages of high sensitivity, high selectivity, fast and easy operation.
出处
《分析试验室》
CAS
CSCD
北大核心
2005年第8期55-57,共3页
Chinese Journal of Analysis Laboratory
