子宫内膜异位症相关性不孕症患者卵泡液TGF-β_1的测定和临床意义被引量：2

TGF-β_1 Levels in Follicular Fluid of Patients with Endometriosis-associated Infertility

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摘要目的探讨子宫内膜异位症(内异症)相关性不孕症患者卵泡液中转化生长因子1β(TGF-β1)测定在诊治中的临床价值。方法酶联免疫吸附分析法(ELISA)测定35例内异症相关性不孕症患者、45例输卵管因素和男性因素不孕症患者,在控制性超排卵(COH)周期取卵日的卵泡液TGF-1β水平,并随访两组的妊娠结局。结果35例内异症组患者的TGF-1β水平(896.2±203.7)pg/mL,与对照组(1005.0±305.7)pg/mL相比较无统计学差异(P>0.05)。内异症组患者的妊娠率与对照组相比显著降低(22.6%versus 37.5%,P<0.001),难免流产率有显著增高(33.3%versus 6.6%,P<0.0001)。结论COH周期排卵日卵泡液的TGF-1β测定对内异症相关性不孕症患者的IVF妊娠结局无辅助诊断价值。 Objective To assess the levels of transforming growth factor-β1 （TGF-β1） in follicular fluid （FF） of patients with endometriosis-associated infertility. Methods TGF-β1 levels were measured by enzymelinked immunosorbent assay （ELISA）in FF samples from 35 subjects with surgically confirmed endometrio- sis and 45 controls. All FF samples were collected on the day of ovum pick-up during controlled ovarian hyperstimulation （COH） cycles while undergoing In vitro fertilization （IVF）. The ultrasonography was performed to conform clinical pregnancies 4 weeks after embryo transfer. Results Levels of TGF-β1 in FF of women with endometriosis-associated infertility had no significant differences compared to controls （896.2± 203.7）pg/mL versus （1005.0±305.7）pg/mL. Pregnancy rate of women with endometriosis-associated infertility was significantly lower than controls （22.6% versus 37.5%, P〈0.001） while missed abortion rate of women with endometriosis-associated infertility was significantly higher than controls （33.3% versus 6.6%, P〈 0.0001 ）. Conclusions The mean concentrations of TGF-β1 in FF of patients with endometriosis-associated infertility could not be used to predict the IVF outcome.

作者许泓钱羽力徐键黄荷凤

机构地区浙江大学医学院附属妇产科医院

出处《科技通报》 2005年第5期561-563,共3页 Bulletin of Science and Technology

基金浙江省医药卫生科学研究基金项目(2004A058)

关键词子宫内膜异位症不孕症卵泡液 TGF—β1 妊娠率 endometriosis infertility follicular fluid TGF-β1 pregnancy rate

分类号 R711.71 [医药卫生—妇产科学]

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1Olive D L, Schwartz L B, Endometriosis[J]. N Engl J Med 1993, 328:1759-69.
2Brosens I, Endometriosis and the outcome of in vitro fertilization[J]. Fertil Steril 2004, 81, 1198-200.
3Nathan C F. Secretory products of macrophages[J]. J Clin Invest 1987, 79, 319-26.
4Mulheron G W, Bossert N L, Lapp J A, et al. Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA [J], J Clin Endocrinol Metab 1992, 74, 458-60.
5Wardle P G, Mitchell J D, McLaughlin E A, et al. Endometriosis and ovulatory disorder: reduced fertilisation in vitro compared with tubal and unexplained infertility[J]. Lancet 1985, 2, 236-9.
6Mahutte N G, Kayisli U, Arici A. Endometriosis is an inflammatory disease[A]. In: Endometriosis in Clinical Practice[C]. London, 2004, 79-88.
7Toya M, Saito H, Ohta N, et al. Moderate and severe endometriosis is associated with alterations in the cell cycle of granulosa cells in patients undergoing in vitro fertilization and embryo transfer[J]. Fertil Steril 2000, 73,344-50.
8Pellicer A, Albert C, Mercader A, et al. The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production [J]. Fertil Steril 1998, 70, 425-31.
9Carlberg M, Nejaty J, Froysa B, et al. Elevated expression of tumour necrosis factor alpha in cultured granulosa cells from women with endometriosis[J]. Hum Reprod 2000, 15, 1250-5.
10Oosterlynck DJ, Meuleman C, Waer M, et al. Transforming growth factor-beta activity is increased in peritoneal fluid from women with endometriosis[J]. Obstet Gynecol 1994, 83, 287-92.