摘要
目的建立Wistar大鼠大脑皮质发育障碍的动物模型。方法采用γ-射线照射孕15d Wistar大鼠的方法制作皮质发育障碍动物模型。观察:(1)孕鼠后代皮质发育障碍的类型和发生率,病理检查大鼠大脑皮质和海马结构;(2)模型组和正常对照组大鼠日常活动能力及脑电图变化;(3)利用热水浴诱导惊厥发作,观察潜伏期;(4)采用Morris水迷宫法测试大鼠学习能力和空间记忆能力。结果(1)模型组大鼠脑重量(932mg)低于正常对照组大鼠(1 300mg,P<0.05),病理可见模型组大鼠脑皮质变薄,皮质层状结构紊乱,皮质下神经元呈结节状异位,海马锥体神经元团状分布;(2)模型鼠日常活动能力较差;(3)模型组热水浴诱导惊厥发作的潜伏期缩短〔两组大鼠分别为(3.65±0.44)min、(4.66±0.58)min,P<0.05〕;(4)模型组水迷宫实验中寻找水下平台时间延长(P<0.05)。结论用γ-射线照射胚胎15d大鼠可建立皮质发育障碍动物模型,其惊厥发作易感性增加,伴有认知功能障碍。
Objective To establish animal models in disorders of cortical development （DCDs） in Wistar rats. Methods Animal models in DCDs are γ-radiated pregnant rats. The rats were divided into model groups and normal control groups. Before EEG recordings, daily activities of rats were observed. Rats were induced seizures by warm-water-immersion（45℃ ）. The memory capacity was tested by using a Morris-water-maze method. Neuronal changes in hippocampus and cerebral cortex were detected by Nissl stain. Results （1）Model rat showed a thin cortical plate and distinct clusters of neuronal elements that represent heterotopias. In the control rat cortex, the typical six-layered lamination pattern is apparent, whereas the model rat cortex shows laminar disorganization and thinning. In addition,there are clusters of large neurons in both superficial and deep layers in the model rat cortex. （2） Animal model groups had less activities than the normal control group. （3）The latent period that warm-water-immerse induced the seizures was significantly shorter than the control（P〈0.05）. （4）The searching platform latency of model rats was significantly longer than that of the normal controls（P〈0. 05）. Conclusion Animal models of DCDs established by γ-radiation decrease seizure threshold and increase seizure susceptibility as well as cognitive handicap.
出处
《重庆医学》
CAS
CSCD
2005年第9期1352-1354,共3页
Chongqing medicine
基金
重庆市教委(渝教科(2001)12-29)
重庆市卫生局资助课题(01-1-013)
关键词
皮质发育障碍
动物模型
难治性癫痫
认知功能
disorders of cortical development
animal models
intractable epilepsy
cognitive function
