摘要
目的研究甘珀酸钠苦参素包合物急性毒性及对CCl4肝损伤小鼠的保护作用。方法采用序贯法,静脉注射测定药物的LD50。采用CCl4制备小鼠急性肝损伤模型,检测经不同比例甘珀酸钠苦参素包合物干预后小鼠血清谷草转氨酶(GOT)、谷丙转氨酶(GPT),取肝脏组织做HE染色常规光镜观察。结果甘珀酸钠苦参素1∶1、1∶22、∶1包合物的急性毒性均比其前体药物甘珀酸钠、苦参素低;与CCl4模型组比较,经甘珀酸钠苦参素1∶12、∶1包合物处理的小鼠血清GOT、GPT水平较低(P<0.05,或P<0.01);小鼠肝脏组织学检查结果显示,甘珀酸钠苦参素2∶1包合物组肝脏形态有明显改善。结论甘珀酸钠与氧化苦参碱不同比例包合物的毒性比其前体药物低,对小鼠急性CCl4肝损伤有保护作用。
Objective To study the acute toxicity of clathrate compound of carbenoxolone and oxymatrine and its protectire effects on injured liver induced by CCl4 in mice. Methods The Sequence Method was employed to test LD50 of the clathrate compound injected intravenously. The GOT and GFF in senun were measured in acute hepatic injury models by CCl4 proportionally injected the clathrate compound intramuscularly in mice. The hepatic structures were observed under microscope. Results The acute toxicity of LD50 of the clathrate compound in 1 ： 1,1 ： 2 and 2： 1 were lower than that of both carbenoxolone sodium and oxymatrine. Compared with the model group by CCl4, the GOT and GFF levels in serum were lower in the clathrate compound of 1 ： 1 and 2 ：1（P 〈 0.05, P 〈 0.01）. The clathrate compound in 2： 1 presented obvious effects in hepatic morphology. Conclusions The toxicity of the clathrate compound in different proportion decreased, compared with their pre. The proportion of 2： 1 presents protection from hepatic injury by CCl4 in mice.
出处
《宁夏医学院学报》
2005年第4期272-273,F0003,共3页
Journal of Ningxia Medical College
关键词
甘珀酸钠
苦参素
包合物
肝损伤
LD50
carbenoxolone
clathrate compound
oxymatrine
hepatic damage
LD50
