摘要
目的通过对苦参碱抗免疫性肝损伤作用的研究，为中药治疗免疫性肝损伤开辟新的途径．方法将大鼠随机分为正常对照组、实验对照组、丹参对照组、苦参碱治疗１组和苦参碱治疗２组，后４组大鼠给猪血清ｉｐ，７ｗｋ以复制肝损伤动物模型，然后分别利用丹参注射液和苦参碱注射液对大鼠进行治疗，共４ｗｋ．实验起１１ｗｋ后处死动物，经心脏取血，离心制备血清，检测血清中ＡＬＴ，ＢＵＮ含量，Ａ／Ｇ值，ＬＮ，ＨＡ含量，ＳＯＤ的活性和ＭＤＡ的含量，剖腹取肝组织制作光、电镜标本检查．结果实验对照组大鼠血清ＡＬＴ，ＢＵＮ，Ａ／Ｇ，ＬＮ，ＨＡ，ＳＯＤ，ＭＤＡ较正常对照组有显著性差异（Ｐ＜００５）；而丹参组和苦参碱组大鼠血清生化指标较正常组无显著性差异（Ｐ＞００５）；与实验对照组相比具有显著性差异（Ｐ＜００５）．光镜下观察：实验对照组大鼠与正常对照组相比，肝细胞受损，胶原纤维面密度远远大于其他组；而丹参组和苦参碱治疗２组与实验对照组相比，肝细胞损伤不明显；丹参和苦参碱组间质细胞数目明显低于实验对照组（Ｐ＜００５）．电镜下观察：丹参和苦参碱组与实验对照组相比较，肝细胞变形很少，线粒体膜未见损伤，细胞质内粗面内质网形态与正常对照组近似，基质内胶原纤?
AIM To further explore possible ways for the treatment of immune liver injury by traditional Chinese medicine matrine (M). METHODS Rats were administrated intra abdominally swine serum to induce immune liver injury model and then were treated with tanshinol (T) and matrine (M) respectively. Serum ALT, BUN, A/G , LN, HA, SOD and MDA were measured and liver specimens were taken and prepared for light and electron microscopic examination. RESULTS The above serum parameters in T or M group had no marked differences from those of normal control group, yet, significant differences did exist between tanshinol (T) or matrine (M) group and experimental control group ( P <0 05). Light and electron microscopic examination showed that, compared with experimental control (EC), no obvious pathological changes were found in the livers of T and M groups and proliferation of collagen fibers greatly decreased too. It was also found that the numbers of interstitial cells were significantly lowered in T and M groups than in EC group. CONCLUSION Matrine had the effect of anti immune liver injury in rats.
出处
《世界华人消化杂志》
CAS
1999年第2期104-108,共5页
World Chinese Journal of Digestology
关键词
肝疾病
病理学
治疗
免疫学
苦参碱
中医药疗法
liver disease/pathology
liver disease/therapy
liver disease/immunology
matrine/therapeutic use
