摘要
在人参（Ｐａｎａｘｇｉｎｓｅｎｇ）细胞悬浮培养中，以无离子水代替重蒸馏水，细胞生长速率和寡糖素产率分别降低２．３％和２．９％。用白糖代替蔗糖，细胞生长速率和寡糖素产率分别降低１．７４％和１．２３％，综合上述两方面结果，以无离子水和白糖分别替代原培养基中的重蒸馏水和蔗糖组成替代培养基，用替代培养基培养人参培养细胞，其生长速率可达０．５０９ｇＤＷ／Ｌ．ｄ，寡糖素产率可达１．４４３９／Ｌ，和原培养基相比，仅分别降低１．７４％和１．２３％，但每吨培养基的成本可以降低近２８００元。用替代培养基培养细胞不改变细胞生长的时间进程，用于寡糖素制备的细胞最佳收获期在２０ｄ左右。
in cell suspension culture of Panax ginseng C. A. Mey, when non-ionic water was used instead of double distilled water, cell growth rate-and oligosaccharin yield were decreased about 2.3% and 2.9% against controlled, respectively, when defined sugar was,used instead of sucrose, cell growth rate and oliogsaccharin yield,were decreased about 1.74% and 1.23% against controlled, respectively. As a result, substitutive medium involving non-ionic water and defined sugar was used instead of original medium in involving redistilled water and sucrose. When cells were suspended in the substitutive, cell growth rate. and oligosaccharin yield were 0.509 gDW / L.d and 1 .443g / L,respectively, which were slightly decreased by 1 .74% and 1 .23% as compared with control in cell suspension culture, while the cost of medium could be largely reduced about 3000 yuan/ t medium. Time course of cell suspension Culture in substitutive medium was been unchanged, an appropriate period for oligosaccharn preparation from P. ginseng cultured cells was about 20 day.
出处
《云南植物研究》
CSCD
1996年第2期139-144,共6页
Acta Botanica Yunnanica
基金
云南省科委"八五"重点攻关项目
关键词
人参
细胞培养
寡糖素
成本降低
Panax ginseng, Cell culture, Oligosaccharin, Cost reduction
